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OxiVision™ Green hydrogen peroxide sensor

Fluorescence responses of OxiVision Green™ to various ROS species.
Fluorescence responses of OxiVision Green™ to various ROS species.
Fluorescence responses of OxiVision Green™ to various ROS species.
Detection of hydrogen peroxide in Jurkat cells. Cells were stained with OxiVision Green™ peroxide sensor for 30 minutes and treated with 100 µM hydrogen peroxide at 37 °C for 90 minutes. Cells stained with OxiVision Green™ peroxide sensor but without hydrogen peroxide treatment were used as control.
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Physical properties
Molecular weight~600
SolventDMSO
Spectral properties
Excitation (nm)498
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
~600
Excitation (nm)
498
Emission (nm)
517
Despite the importance of H2O2 to human health and disease, the molecular mechanisms of its production, accumulation, trafficking, and function are insufficiently understood due to the lack of sensitive and specific H2O2 sensors that can be used in live cells. The limitations of currently available H2O2-responsive probes include interfering background fluorescence from other ROS, the need for an external activating enzyme, lack of water solubility or compatibility, and/or excitation profiles in the ultraviolet region. OxiVision Green™ hydrogen peroxide sensor is non-fluorescent and displays no absorption in the visible region. The addition of H2O2 triggers a prompt fluorescence increase with concomitant growth of a visible wavelength absorption band. This probe has a large dynamic range owing to its binary absorption/emission response. The fluorescence response of OxiVision Green™ hydrogen peroxide sensor is H2O2-selective. OxiVision Green™ hydrogen peroxide sensor exhibits a >100-fold selectivity for H2O2 over similar ROS such as O2-, NO, or -OCl.

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Spectral properties

Excitation (nm)498
Emission (nm)517

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Citations


View all 13 citations: Citation Explorer
Rational design of ICD-inducing nanoparticles for cancer immunotherapy
Authors: Zhang, Zhanzhan and Pan, Zheng and Li, Qiushi and Huang, Qingqing and Shi, Linqi and Liu, Yang
Journal: Science Advances (2024): eadk0716
Superoxide Dismutase-like Activity of Zeolitic Imidazolate Framework Nanoparticles Comprising Biomimetic Imidazolato-bridged CuZn Units
Authors: Nakahara, Hiroki and Nomura, Akiko and Tokuda, Shun and Okamura, Mami and Fujisawa, Kiyoshi and Koitaya, Takanori and Yoshida, Yasuhiro and Furukawa, Shuhei and Hitomi, Yutaka
Journal: Chemistry--A European Journal (2023): e202300881
Mitochondrial Dysfunction and Antioxidation Dyshomeostasis-Enhanced Tumor Starvation Synergistic Chemotherapy Achieved using a Metal--Organic Framework-Based Nano-Enzyme Reactor
Authors: Huo, Taotao and Leilei chen, and Nie, Huifang and Li, Wenshuai and Lin, Chenteng and Akhtar, Muhammad and Huang, Rongqin
Journal: ACS Applied Materials \& Interfaces (2022)
Plasma-activated Ringer's lactate solution inhibits the cellular respiratory system in HeLa cells
Authors: Tanaka, Hiromasa and Maeda, Shogo and Nakamura, Kae and Hashizume, Hiroshi and Ishikawa, Kenji and Ito, Mikako and Ohno, Kinji and Mizuno, Masaaki and Motooka, Yashiro and Okazaki, Yasumasa and others,
Journal: Plasma Processes and Polymers (2021): e2100056
Biodegradable Hollow Manganese Silicate Nanocomposites to Alleviate Tumor Hypoxia toward Enhanced Photodynamic Therapy
Authors: Nafiujjaman, Md and Chung, Seock-Jin and Kalashnikova, Irina and Hill, Meghan L and Homa, Silver and George, Jeron and Contag, Christopher H and Kim, Taeho
Journal: ACS Applied Bio Materials (2020): 7989--7999
ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils
Authors: Wang, Duo and Sennari, Yusuke and Shen, Mengyue and Morita, Kentaro and Kanazawa, Tamotsu and Yoshida, Yasuhiro
Journal: International Immunopharmacology (2020): 106510
In situ Oxygenic Nanopods Targeting Tumor Adaption to Hypoxia Potentiate Image-guided Photothermal Therapy
Authors: Revuri, Vishnu and Cherukula, Kondareddy and Nafiujjaman, Md and Vijayan, Veena and Jeong, Yong Yeon and Park, In-Kyu and Lee, Yong-kyu
Journal: ACS applied materials &amp; interfaces (2019)
Semaphorin 4D inhibits neutrophil activation and is involved in the pathogenesis of neutrophil-mediated autoimmune vasculitis
Authors: Nishide, Masayuki and Nojima, Satoshi and Ito, Daisuke and Takamatsu, Hyota and Koyama, Shohei and Kang, Sujin and Kimura, Tetsuya and Morimoto, Keiko and Hosokawa, Takashi and Hayama, Yoshitomo and others, undefined
Journal: Annals of the Rheumatic Diseases (2017): annrheumdis--2016
Hydrogen peroxide detection with high specificity in living cells and inflamed tissues
Authors: Rong, Lei and Zhang, Chi and Lei, Qi and Hu, Ming-Ming and Feng, Jun and Shu, Hong-Bing and Liu, Yi and Zhang, Xian-Zheng
Journal: Regenerative Biomaterials (2016): rbw022
Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging
Authors: Uemura, Takeshi and Watanabe, Kenta and Ishibashi, Misaki and Saiki, Ryotaro and Kuni, Kyoshiro and Nishimura, Kazuhiro and Toida, Toshihiko and Kashiwagi, Keiko and Igarashi, Kazuei
Journal: Biochemical and biophysical research communications (2016): 630--635

References


View all 54 references: Citation Explorer
Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
Authors: Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, Terskikh AV, Lukyanov S.
Journal: Nat Methods (2006): 281
Fluorescent quenching method for determination of trace hydrogen peroxide in rain water
Authors: Chen H, Yu H, Zhou Y, Wang L.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system
Authors: Almeida LE, Imasato H, Tabak M.
Journal: Biochim Biophys Acta (2006): 216
Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes
Authors: Bienert GP, Moller AL, Kristiansen KA, Schulz A, Moller IM, Schjoerring JK, Jahn TP.
Journal: J Biol Chem. (2006)
Nonenzymatic direct assay of hydrogen peroxide at neutral pH using the Eu3Tc fluorescent probe
Authors: Durkop A, Wolfbeis OS.
Journal: J Fluoresc (2005): 755
Hydroxyl radical formation resulting from the interaction of nickel complexes of L-histidine, glutathione or L-cysteine and hydrogen peroxide
Authors: Joshi S, Husain MM, Ch and ra R, Hasan SK, Srivastava RC.
Journal: Hum Exp Toxicol (2005): 13
Oxidation of a eukaryotic 2-Cys peroxiredoxin is a molecular switch controlling the transcriptional response to increasing levels of hydrogen peroxide
Authors: Bozonet SM, Findlay VJ, Day AM, Cameron J, Veal EA, Morgan BA.
Journal: J Biol Chem (2005): 23319
Shift in the localization of sites of hydrogen peroxide production in brain mitochondria by mitochondrial stress
Authors: Gyulkh, undefined and anyan AV, Pennefather PS.
Journal: J Neurochem (2004): 405
Cremophor EL augments the cytotoxicity of hydrogen peroxide in lymphocytes dissociated from rat thymus glands
Authors: Iwase K, Oyama Y, Tatsuishi T, Yamaguchi JY, Nishimura Y, Kanada A, Kobayashi M, Maemura Y, Ishida S, Okano Y.
Journal: Toxicol Lett (2004): 143
Protection against hydrogen peroxide-induced cytotoxicity in PC12 cells by scutellarin
Authors: Hong H, Liu GQ.
Journal: Life Sci (2004): 2959