Thiolite™ Green

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	~400
Solvent	DMSO

Spectral properties

Excitation (nm)	505
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

Overview SDS Protocol

Molecular weight

~400

Excitation (nm)

505

Emission (nm)

524

Thiolite™ Green is one of the most sensitive sensors for measuring thiol compounds. It gives a green fluorescent adduct upon reacting with thiol compounds (such as cysteine). It can be used to quantifying the number of cysteines on a protein. We have used it to measure glutathione fluorimetrically. It has >200-fold fluorescence enhancement upon reaction with thiol-containing compounds.

Spectrum

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Spectral properties

Excitation (nm)	505
Emission (nm)	524

Product Family

Name	Excitation (nm)	Emission (nm)
Thiolite™ Blue	335	460

Images

Figure 1. Spectrum for Thiolite Green

Citations

View all 8 citations: Citation Explorer

Carboranyl-1, 8-naphthalimide intercalators induce lysosomal membrane permeabilization and ferroptosis in cancer cell lines
Authors: Rykowski, Sebastian and Gurda-Wo{\'z}na, Dorota and Fedoruk-Wyszomirska, Agnieszka and Orlicka-P{\l}ocka, Marta and Kowalczyk, Aleksandra and St{\k{a}}czek, Pawe{\l} and Denel-Bobrowska, Marta and Biniek-Antosiak, Katarzyna and Rypniewski, Wojciech and Wyszko, Eliza and others,
Journal: Journal of Enzyme Inhibition and Medicinal Chemistry (2023): 2171028

Implications of Oxidative Stress in Glioblastoma Multiforme Following Treatment with Purine Derivatives
Authors: Orlicka-P{\l}ocka, Marta and Fedoruk-Wyszomirska, Agnieszka and Gurda-Wo{\'z}na, Dorota and Pawelczak, Pawe{\l} and Krawczyk, Patrycja and Giel-Pietraszuk, Ma{\l}gorzata and Framski, Grzegorz and Ostrowski, Tomasz and Wyszko, Eliza
Journal: Antioxidants (2021): 950

Revealing 3D structure of gluten in wheat dough by optical clearing imaging
Authors: Ogawa, Takenobu and Matsumura, Yasuki
Journal: Nature communications (2021): 1--10

Circumventing the Crabtree effect: forcing oxidative phosphorylation (OXPHOS) via galactose medium increases sensitivity of HepG2 cells to the purine derivative kinetin riboside
Authors: Orlicka-P{\l}ocka, Marta and Gurda-Wozna, Dorota and Fedoruk-Wyszomirska, Agnieszka and Wyszko, Eliza
Journal: Apoptosis (2020): 835--852

Quercetin Triggers Induction of Apoptotic and Lysosomal Death of Sensitive and Multidrug Resistant Leukaemia HL60 Cells
Authors: Maruszewska, Agnieszka and Tarasiuk, Jolanta
Journal: Nutrition and Cancer (2020): 1--18

The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function
Authors: Wu, Tsung-Han and Yeh, Kun-Yun and Wang, Chen-Hsu and Wang, Hang and Li, Tsung-Lin and Chan, Yi-Lin and Wu, Chang-Jer
Journal: Journal of oncology (2019)

Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans
Authors: Xiao, Man and Du, Guankui and Zhong, Guobing and Yan, Dongjing and Zeng, Huazong and Cai, Wangwei
Journal: PloS one (2016): e0151103

Virus-like particles with removable cyclodextrins enable glutathione-triggered drug release in cells
Authors: Niikura, Kenichi and Sugimura, Naotoshi and Musashi, Yusuke and Mikuni, Shintaro and Matsuo, Yasutaka and Kobayashi, Shintaro and Nagakawa, Keita and Takahara, Shuko and Takeuchi, Chie and Sawa, Hirofumi and others, undefined
Journal: Molecular biosystems (2013): 501--507

References

View all 74 references: Citation Explorer

Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
Authors: Meziane D, Barras A, Kromka A, Houdkova J, Boukherroub R, Szunerits S.
Journal: Anal Chem (2012): 194

Simultaneous Nucleophilic-substituted and Electrostatic Interactions for Thermal Switching of Spiropyran: A New Approach for Rapid and Selective Colorimetric Detection of Thiol-containing Amino Acids
Authors: Li Y, Duan Y, Li J, Zheng J, Yu H, Yang R.
Journal: Anal Chem. (2012)

A sensitive and rapid ultra HPLC-MS/MS method for the simultaneous detection of clopidogrel and its derivatized active thiol metabolite in human plasma
Authors: Peer CJ, Spencer SD, VanDenBerg DA, Pacanowski MA, Horenstein RB, Figg WD.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2012): 132

Electrochemical detection of celiac disease-related anti-tissue transglutaminase antibodies using thiol based surface chemistry
Authors: Dulay S, Lozano-Sanchez P, Iwuoha E, Katakis I, O'Sullivan CK.
Journal: Biosens Bioelectron. (2011)

A microchip electrophoresis strategy with online labeling and chemiluminescence detection for simultaneous quantification of thiol drugs
Authors: Huang Y, Zhao S, Shi M, Liang H.
Journal: J Pharm Biomed Anal (2011): 889

Investigation of chemically modified barium titanate beads as surface-enhanced Raman scattering (SERS) active substrates for the detection of benzene thiol, 1,2-benzene dithiol, and rhodamine 6G
Authors: Onuegbu J, Fu A, Glembocki O, Pokes S, Alexson D, Hosten CM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2011): 456

Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition
Authors: Lin Y, Tao Y, Ren J, Pu F, Qu X.
Journal: Biosens Bioelectron (2011): 339

Modulating DNA-templated silver nanoclusters for fluorescence turn-on detection of thiol compounds
Authors: Huang Z, Pu F, Lin Y, Ren J, Qu X.
Journal: Chem Commun (Camb) (2011): 3487

Thiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS
Authors: Lambert W, Soderberg CA, Rutsdottir G, Boelens WC, Emanuelsson C.
Journal: Protein Sci (2011): 1682

Indicator approach to develop a chemosensor for the colorimetric sensing of thiol-containing water and its application for the thiol detection in plasma
Authors: Huo FJ, Yang YT, Su J, Sun YQ, Yin CX, Yan XX.
Journal: Analyst (2011): 1892