Fluorescein dicaprylate [Fluorescein dioctanoate]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	584.70
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein dicaprylate [Fluorescein dioctanoate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.028 µL	855.139 µL	1.71 mL	8.551 mL	17.103 mL
5 mM	34.206 µL	171.028 µL	342.056 µL	1.71 mL	3.421 mL
10 mM	17.103 µL	85.514 µL	171.028 µL	855.139 µL	1.71 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein DHPE	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein aldehyde [5-FAM aldehyde]	493	517	83000	-	0.32	0.178
Fluorescein Tyramide	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein dicaprylate [Fluorescein dioctanoate]

Citations

View all 4 citations: Citation Explorer

Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Authors: Xu, Yufan and Wang, Xiaohong
Journal: Biotechnology and bioengineering (2015): 1683--1695

Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses
Authors: Yin, Yan-Xu and Wang, Shu-Bin and Xiao, Huai-Juan and Zhang, Huai-Xia and Zhang, Zhen and Jing, Hua and Zhang, Ying-Li and Chen, Ru-Gang and Gong, Zhen-Hui
Journal: International journal of molecular sciences (2014): 20101--20116

Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
Authors: Obiakor, Harold and Avril, Marion and MacDonald, Nicholas J and Srinivasan, Prakash and Reiter, Karine and Anderson, Charles and Holmes, Kevin L and Fried, Michal and Duffy, Patrick E and Smith, Joseph D and others, undefined
Journal: Clinical and Vaccine Immunology (2013): 433--442

Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function
Authors: Minematsu, Takeo and Yamamoto, Yuko and Nagase, Takashi and Naito, Ayumi and Takehara, Kimie and Iizaka, Shinji and Komagata, Kazunori and Huang, Lijuan and Nakagami, Gojiro and Akase, Tomoko and others, undefined
Journal: Journal of dermatological science (2011): 160--168

References

View all 68 references: Citation Explorer

Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester
Authors: Terho P, Lassila O.
Journal: Cytometry A (2006): 552

Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods
Authors: Brouwer N, Kohen J, Jamie J, Vemulpad S.
Journal: J Microbiol Methods (2006): 234

Optimisation of the fluorescein diacetate antibacterial assay
Authors: Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.
Journal: J Microbiol Methods (2005): 21

A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes
Authors: Peng XY, Li PC.
Journal: Anal Chem (2004): 5282

Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling
Authors: Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.
Journal: Transplantation (2003): 1075

Carboxy-fluorescein diacetate, succinimidyl ester labeled papillomavirus virus-like particles fluoresce after internalization and interact with heparan sulfate for binding and entry
Authors: Drobni P, Mistry N, McMillan N, Ev and er M., undefined
Journal: Virology (2003): 163

Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Authors: Lomolino G, Lante A, Crapisi A, Spettoli P, Curioni A.
Journal: Electrophoresis (2001): 1021

Fluorescein diacetate hydrolysis as a measure of fungal biomass in soil
Authors: Gaspar ML, Cabello MN, Pollero R, Aon MA.
Journal: Curr Microbiol (2001): 339

Extrusion of fluorescein diacetate by multidrug-resistant Candida albicans
Authors: Yang HC, Mikami Y, Imai T, Taguchi H, Nishimura K, Miyaji M, Branchini ML.
Journal: Mycoses (2001): 368

Potential problems with fluorescein diacetate assays of cell viability when testing natural products for antimicrobial activity
Authors: Clarke JM, Gillings MR, Altavilla N, Beattie AJ.
Journal: J Microbiol Methods (2001): 261