DiD Perchlorate *UltraPure Grade*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
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International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 959.91 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 646 |
Emission (nm) | 663 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Plasma Membrane
CAS 127274-91-3 | Molecular weight 959.91 | Extinction coefficient (cm -1 M -1) 260000 | Excitation (nm) 646 | Emission (nm) 663 |
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiD Perchlorate *UltraPure Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 104.176 µL | 520.882 µL | 1.042 mL | 5.209 mL | 10.418 mL |
5 mM | 20.835 µL | 104.176 µL | 208.353 µL | 1.042 mL | 2.084 mL |
10 mM | 10.418 µL | 52.088 µL | 104.176 µL | 520.882 µL | 1.042 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 646 |
Emission (nm) | 663 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* | 483 | 501 | - |
DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
Citations
View all 53 citations: Citation Explorer
Observation of antitumor mechanism of GE11-modified paclitaxel and curcumin liposomes based on cellular morphology changes
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
Authors: Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,
Journal: AAPS Open (2024): 1
An oncolytic virus--T cell chimera for cancer immunotherapy
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Authors: Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan
Journal: Nature Biotechnology (2024): 1--12
Two-Pronged Intracellular Co-Delivery of Antigen and Adjuvant for Synergistic Cancer Immunotherapy
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Authors: Meng, Junli and Zhang, Peisen and Chen, Qizhe and Wang, Zihua and Gu, Yuan and Ma, Jie and Li, Wang and Yang, Chen and Qiao, Yuanyuan and Hou, Yi and others,
Journal: Advanced Materials (2022): 2202168
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Directional Migration and Odontogenic Differentiation of Bone Marrow Stem Cells Induced by Dentin Coated with Nanobioactive Glass
Authors: Wang, Sainan and Huang, Guibin and Dong, Yanmei
Journal: Journal of Endodontics (2019)
Authors: Wang, Sainan and Huang, Guibin and Dong, Yanmei
Journal: Journal of Endodontics (2019)
The Influence of Cell Source and Donor Age on the Tenogenic Potential and Chemokine Secretion of Human Mesenchymal Stromal Cells
Authors: Zarychta-Wisniewska, Weronika and Burdzińska, Anna and Zielniok, Katarzyna and Koblowska, Marta and Gala, Kamila and Pedzisz, Piotr and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Aksamit, Aleks and ra , undefined and Kulesza, Agnieszka and others, undefined
Journal: Stem Cells International (2019)
Authors: Zarychta-Wisniewska, Weronika and Burdzińska, Anna and Zielniok, Katarzyna and Koblowska, Marta and Gala, Kamila and Pedzisz, Piotr and Iwanicka-Nowicka, Roksana and Fogtman, Anna and Aksamit, Aleks and ra , undefined and Kulesza, Agnieszka and others, undefined
Journal: Stem Cells International (2019)
Loss of Integrity of Doxorubicin Liposomes during Transcellular Transportation Evidenced by Fluorescence Resonance Energy Transfer Effect
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
Intraurethral co-transplantation of bone marrow mesenchymal stem cells and muscle-derived cells improves the urethral closure
Authors: Burdzinska, Anna and Dybowski, Bartosz and Zarychta-Wisniewska, Weronika and Kulesza, Agnieszka and Butrym, Marta and Zagozdzon, Radoslaw and Graczyk-Jarzynka, Agnieszka and Radziszewski, Piotr and Gajewski, Zdzislaw and Paczek, Leszek
Journal: Stem cell research & therapy (2018): 239
Authors: Burdzinska, Anna and Dybowski, Bartosz and Zarychta-Wisniewska, Weronika and Kulesza, Agnieszka and Butrym, Marta and Zagozdzon, Radoslaw and Graczyk-Jarzynka, Agnieszka and Radziszewski, Piotr and Gajewski, Zdzislaw and Paczek, Leszek
Journal: Stem cell research & therapy (2018): 239
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
References
View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
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Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?