JC-10 *Superior alternative to JC-1*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 583.34 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 508 |
Emission (nm) | 524 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Mitochondria, Mitochondrial Membrane Potential
Molecular weight 583.34 | Excitation (nm) 508 | Emission (nm) 524 |
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be an alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized. Both colors can be detected using the filters commonly mounted in all flow cytometers, so that green emission can be analyzed in fluorescence channel 1 (FL1) and greenish orange emission in channel 2 (FL2). Besides its potential use in flow cytometry, it can also be used in fluorescence imaging. We have developed a protocol to use JC-10 in fluorescence microplate platform. In some cell lines JC-10 has even superior performance to JC-1. Interestingly the performance of JC-10 is quite cell line-dependent. Our JC-10 is conveniently provided in DMSO solution at ~3 mM concentration (2 mg/mL).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of JC-10 *Superior alternative to JC-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 171.427 µL | 857.133 µL | 1.714 mL | 8.571 mL | 17.143 mL |
5 mM | 34.285 µL | 171.427 µL | 342.853 µL | 1.714 mL | 3.429 mL |
10 mM | 17.143 µL | 85.713 µL | 171.427 µL | 857.133 µL | 1.714 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
Images
Figure 1. Campotothecin-induced mitochondria membrane potential changes were measured with JC-10™ and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 µM) for 4 hours, JC-1 and JC-10™ dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10™ were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader (BMG Labtech).
Citations
View all 65 citations: Citation Explorer
Molecular mechanisms of naringenin modulation of mitochondrial permeability transition acting on F1FO-ATPase and counteracting saline load-induced injury in SHRSP cerebral endothelial cells
Authors: Nesci, Salvatore and Algieri, Cristina and Tallarida, Matteo Antonio and Stanzione, Rosita and Marchi, Saverio and Pietrangelo, Donatella and Trombetti, Fabiana and D’Ambrosio, Luca and Forte, Maurizio and Cotugno, Maria and others,
Journal: European Journal of Cell Biology (2024): 151398
Authors: Nesci, Salvatore and Algieri, Cristina and Tallarida, Matteo Antonio and Stanzione, Rosita and Marchi, Saverio and Pietrangelo, Donatella and Trombetti, Fabiana and D’Ambrosio, Luca and Forte, Maurizio and Cotugno, Maria and others,
Journal: European Journal of Cell Biology (2024): 151398
Self-heating mitochondrion-induced free radical blast for immunogenic cell death stimulation and HCC immunotherapy
Authors: Peng, Mengyun and Dong, Hongyan and Shao, Meiyu and Zhang, Xiaoqing and Sun, Jiamei and Ding, Chuan and Han, Xin and Yang, Qiao and Sang, Xianan and Cao, Gang
Journal: Journal of Controlled Release (2024): 694--711
Authors: Peng, Mengyun and Dong, Hongyan and Shao, Meiyu and Zhang, Xiaoqing and Sun, Jiamei and Ding, Chuan and Han, Xin and Yang, Qiao and Sang, Xianan and Cao, Gang
Journal: Journal of Controlled Release (2024): 694--711
Mitochondria-synthesize melatonin to improve the quality and in vitro maturation as well as the embryonic development of porcine oocytes
Authors: Zhu, Tianqi and Wang, Likai and Ma, Xiao and Li, Guangdong and Fu, Yao and Ji, Pengyun and Zhang, Lu and Liu, Guoshi and others,
Journal: (2024)
Authors: Zhu, Tianqi and Wang, Likai and Ma, Xiao and Li, Guangdong and Fu, Yao and Ji, Pengyun and Zhang, Lu and Liu, Guoshi and others,
Journal: (2024)
SAP130 released by ferroptosis tubular epithelial cells promotes macrophage polarization via Mincle signaling in sepsis acute kidney injury
Authors: Zhang, Jing and Jiang, Jun and Wang, Bingqing and Wang, Yue and Qian, Yaoyao and Suo, Jinmeng and Li, Yiming and Peng, Zhiyong
Journal: International Immunopharmacology (2024): 111564
Authors: Zhang, Jing and Jiang, Jun and Wang, Bingqing and Wang, Yue and Qian, Yaoyao and Suo, Jinmeng and Li, Yiming and Peng, Zhiyong
Journal: International Immunopharmacology (2024): 111564
Differences in the response of normal oral mucosa, oral leukoplakia, oral squamous cell carcinoma-derived mesenchymal stem cells, and epithelial cells to photodynamic therapy
Authors: Guo, Qianyun and Ji, Xiaoli and Zhang, Lei and Liu, Xingyun and Wang, Yutian and Liu, Zijian and Jin, Jianqiu and Han, Ying and Liu, Hongwei
Journal: Journal of Photochemistry and Photobiology B: Biology (2024): 112907
Authors: Guo, Qianyun and Ji, Xiaoli and Zhang, Lei and Liu, Xingyun and Wang, Yutian and Liu, Zijian and Jin, Jianqiu and Han, Ying and Liu, Hongwei
Journal: Journal of Photochemistry and Photobiology B: Biology (2024): 112907
Sediment pollutant exposures caused hepatotoxicity and disturbed glycogenesis
Authors: Lin, Meng-Wei and Yu, Xin-Ru and Chen, Jai-Yu and Wei, Yu-Shan and Chen, Hsin-Yi and Tsai, Yi-Ting and Lin, Li-Hsun and Liao, En-Chi and Kung, Hsiang-Yu and Young, Shuh-Sen and others,
Journal: Ecotoxicology and Environmental Safety (2023): 114559
Authors: Lin, Meng-Wei and Yu, Xin-Ru and Chen, Jai-Yu and Wei, Yu-Shan and Chen, Hsin-Yi and Tsai, Yi-Ting and Lin, Li-Hsun and Liao, En-Chi and Kung, Hsiang-Yu and Young, Shuh-Sen and others,
Journal: Ecotoxicology and Environmental Safety (2023): 114559
Inhibition of PDGFR$\beta$ alleviates endothelial cell apoptotic injury caused by DRP-1 overexpression and mitochondria fusion failure after mitophagy
Authors: An, Xiaohong and Ma, Xiao and Liu, Heng and Song, Jing and Wei, Tiange and Zhang, Rongzhan and Zhan, Xiao and Li, Hongyang and Zhou, Jia
Journal: Cell Death \& Disease (2023): 1--17
Authors: An, Xiaohong and Ma, Xiao and Liu, Heng and Song, Jing and Wei, Tiange and Zhang, Rongzhan and Zhan, Xiao and Li, Hongyang and Zhou, Jia
Journal: Cell Death \& Disease (2023): 1--17
Atrial natriuretic peptide signaling co-regulates lipid metabolism and ventricular conduction system gene expression in the embryonic heart
Authors: Mishra, Abhishek and Tavasoli, Mahtab and Sokolenko, Stanislav and McMaster, Christopher R and Pasumarthi, Kishore BS
Journal: iScience (2023)
Authors: Mishra, Abhishek and Tavasoli, Mahtab and Sokolenko, Stanislav and McMaster, Christopher R and Pasumarthi, Kishore BS
Journal: iScience (2023)
A hyaluronic acid modified cuprous metal-organic complex for reversing multidrug resistance via redox dyshomeostasis
Authors: Wan, Yichen and Chen, Zelong and Wang, Yi and Zhao, Wenkang and Pei, Zhichao and Pu, Liang and Lv, Yinghua and Li, Jiaxuan and Li, Jiahui and Pei, Yuxin
Journal: Carbohydrate Polymers (2023): 120762
Authors: Wan, Yichen and Chen, Zelong and Wang, Yi and Zhao, Wenkang and Pei, Zhichao and Pu, Liang and Lv, Yinghua and Li, Jiaxuan and Li, Jiahui and Pei, Yuxin
Journal: Carbohydrate Polymers (2023): 120762
Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
Authors: Williamson, Matthew G and Madureira, Marta and McGuinness, William and Heon-Roberts, Rachel and Mock, Elliot D and Naidoo, Kalina and Cramb, Kaitlyn ML and Caiazza, Maria-Claudia and Belen Malpartida, Ana and Lavelle, Martha and others,
Journal: Human Molecular Genetics (2023): ddad102
Authors: Williamson, Matthew G and Madureira, Marta and McGuinness, William and Heon-Roberts, Rachel and Mock, Elliot D and Naidoo, Kalina and Cramb, Kaitlyn ML and Caiazza, Maria-Claudia and Belen Malpartida, Ana and Lavelle, Martha and others,
Journal: Human Molecular Genetics (2023): ddad102
References
View all 6 references: Citation Explorer
Tissue plasminogen activator regulates Purkinje neuron development and survival
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
Authors: Jianxue Li, Lili Yu, Xuesong Gu, Yinghua Ma, Renata Pasqualini, Wadih Arap, Evan Y. Snyder, and Richard L. Sidman, undefined
Journal: PNAS (2013): E2410 - E2419
5,6-Dimethylxanthenone-4-acetic Acid (DMXAA) Activates Stimulator of Interferon Gene (STING)-dependent Innate Immune Pathways and Is Regulated by Mitochondrial Membrane Potential
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Authors: Prantner D, Perkins DJ, Lai W, Williams MS, Sharma S, Fitzgerald KA, Vogel SN
Journal: J Biol Chem (2012): 39776-88
Application of a homogenous membrane potential assay to assess mitochondrial function
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Authors: Sakamuru S, Li X, Attene-Ramos MS, Huang R, Lu J, Shou L, Shen M, Tice RR, Austin CP, Xia M
Journal: Physiol Genomics (2012): 495-503
Human iPS-derived Cardiomyocytes for Cardiotoxicity Screening
Authors: , undefined
Journal: SBS Molecular Devices (2011)
Authors: , undefined
Journal: SBS Molecular Devices (2011)
Analyzing Cellular Apoptosis Through Monitoring Mitochondrial Membrane Potential Changes with JC-10
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
Authors: Jinfang Liao, Qin Zhao, Xing Han, Zhenjun Diwu
Journal: Biophysical Journal : 2
iCell® Cardiomyocytes: Assaying Mitochondrial Membrane Potential
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf
Authors: , undefined
Journal: http://www.cellulardynamics.com/products/lit/CDI_appnote_Cardiomyocytes_mitochondrial.pdf
Application notes
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
High-Content High-Throughput Assays and iPSC Neuronal Cultures
Susceptibility of gametes and embryos of the eastern oyster
Tetrandrine protects mouse retinal ganglion cells from ischemic injury
Midazolam Induces Cellular Apoptosis in Human Cancer Cells
High-Content High-Throughput Assays and iPSC Neuronal Cultures
Susceptibility of gametes and embryos of the eastern oyster
Tetrandrine protects mouse retinal ganglion cells from ischemic injury
Midazolam Induces Cellular Apoptosis in Human Cancer Cells
FAQ
Why is determining cell viability important?
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
AssayWise
Selective Probes for Studying Mitochondrial Functionality
Fluorescent Tools for Studying Mitochondrial Morphology and Function
Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
Nucleic Acid Detection, Quantification and Imaging
A practical guide for use of PE and APC in flow cytometry
Fluorescent Tools for Studying Mitochondrial Morphology and Function
Comprehensive Guide to Cell Health Assays in Flow Cytometry: A Multidimensional Exploration
Nucleic Acid Detection, Quantification and Imaging
A practical guide for use of PE and APC in flow cytometry