DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1*
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Additional ordering information
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 380.27 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Mitochondria
CAS 3785-01-1 | Molecular weight 380.27 |
DASPEI is a styryl dye that stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane. The vital mitochondrial dye DASPEI has been used for the rapid visualization of several distinct classes of epidermal cells in vivo and in vitro: epidermal electroreceptors, mechanoreceptors, and chloride cells in teleosts, and mechanoreceptors in amphibians. DASPEI is also used to locate a different type of sensory cells. In vivo, bath-applied DASPEI stains entire larvae in a relatively non-specific fashion.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 262.971 µL | 1.315 mL | 2.63 mL | 13.149 mL | 26.297 mL |
5 mM | 52.594 µL | 262.971 µL | 525.942 µL | 2.63 mL | 5.259 mL |
10 mM | 26.297 µL | 131.486 µL | 262.971 µL | 1.315 mL | 2.63 mL |
Molarity calculator
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Images
Citations
View all 1 citations: Citation Explorer
Environmental Hazard Potential of Nano-Photocatalysts Determined by Nano-Bio Interactions and Exposure Conditions
Authors: He, Yuan and Peng, Guotao and Jiang, Yue and Zhao, Mei and Wang, Xiaoxiao and Chen, Mengmeng and Lin, Sijie
Journal: Small (2020): 1907690
Authors: He, Yuan and Peng, Guotao and Jiang, Yue and Zhao, Mei and Wang, Xiaoxiao and Chen, Mengmeng and Lin, Sijie
Journal: Small (2020): 1907690
References
View all 17 references: Citation Explorer
Development of a liquid chromatography/mass spectrometry-based drug accumulation assay in Pseudomonas aeruginosa
Authors: Cai H, Rose K, Liang LH, Dunham S, Stover C.
Journal: Anal Biochem (2009): 321
Authors: Cai H, Rose K, Liang LH, Dunham S, Stover C.
Journal: Anal Biochem (2009): 321
Epithelial mitochondria-rich cells and associated innervation in adult and developing zebrafish
Authors: Jonz MG, Nurse CA.
Journal: J Comp Neurol (2006): 817
Authors: Jonz MG, Nurse CA.
Journal: J Comp Neurol (2006): 817
The use of zebrafish for assessing ototoxic and otoprotective agents
Authors: Ton C, Parng C.
Journal: Hear Res (2005): 79
Authors: Ton C, Parng C.
Journal: Hear Res (2005): 79
Mitochondria-rich cell activity in the yolk-sac membrane of tilapia (Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels
Authors: Lin LY, Hwang PP.
Journal: J Exp Biol (2004): 1335
Authors: Lin LY, Hwang PP.
Journal: J Exp Biol (2004): 1335
Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport
Authors: Murakami S, Tamura N, Saito A, Hirata T, Yamaguchi A.
Journal: J Biol Chem (2004): 3743
Authors: Murakami S, Tamura N, Saito A, Hirata T, Yamaguchi A.
Journal: J Biol Chem (2004): 3743
Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio)
Authors: Harris JA, Cheng AG, Cunningham LL, MacDonald G, Raible DW, Rubel EW.
Journal: J Assoc Res Otolaryngol (2003): 219
Authors: Harris JA, Cheng AG, Cunningham LL, MacDonald G, Raible DW, Rubel EW.
Journal: J Assoc Res Otolaryngol (2003): 219
Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio)
Authors: Murakami SL, Cunningham LL, Werner LA, Bauer E, Pujol R, Raible DW, Rubel EW.
Journal: Hear Res (2003): 47
Authors: Murakami SL, Cunningham LL, Werner LA, Bauer E, Pujol R, Raible DW, Rubel EW.
Journal: Hear Res (2003): 47
Calcium ion triggers rapid morphological oscillation of chloride cells in the mudskipper, Periophthalmus modestus
Authors: Sakamoto T, Ando M.
Journal: J Comp Physiol B (2002): 435
Authors: Sakamoto T, Ando M.
Journal: J Comp Physiol B (2002): 435
Mitochondria-rich cells in gills and skin of an African lungfish, Protopterus annectens
Authors: Sturla M, Masini MA, Prato P, Grattarola C, Uva B.
Journal: Cell Tissue Res (2001): 351
Authors: Sturla M, Masini MA, Prato P, Grattarola C, Uva B.
Journal: Cell Tissue Res (2001): 351
Rapid morphological oscillation of mitochondrion-rich cell in estuarine mudskipper following salinity changes
Authors: Sakamoto T, Yokota S, Ando M.
Journal: J Exp Zool (2000): 666
Authors: Sakamoto T, Yokota S, Ando M.
Journal: J Exp Zool (2000): 666
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
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Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?