Cell Explorer™ Live Cell Tracking Kit *Orange Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Platform
Flow cytometer
Excitation | 488 nm or 532 nm laser |
Emission | 575/26 nm filter |
Instrument specification(s) | PE channel |
Fluorescence microscope
Excitation | 540 nm |
Emission | 570 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Cy3/TRITC filter |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples and remove cells from incubator
- Add 10 µL/well of 10X Track It™ Orange working solution in each well
- Stain the cells at 37°C for 15 minutes to 1 hour
- Wash the cells
- Examine the specimen under fluorescence microscope with Cy3/TRITC filter or flow cytometer with 575/26 nm filter (PE channel)
Important notes
Thaw all the components to room temperature, centrifuge the component A briefly before opening.
PREPARATION OF WORKING SOLUTION
Dilute 500X Track It™ Orange DMSO stock solution (Component A) into Assay Buffer (Component B) to make a 10X to 25X Track It™ Orange working solution. The working solution should be prepared enough for all the wells at 10 µL/well with the appropriate concentration. For example, to get a 1X final concentration of Track It™ Orange for one 96-well microplate, dilute 20 µL of the Track It™ Orange DMSO stock solution into 1 mL of Assay Buffer (Component B) to make 1 mL of 10X Track It™ Orange working solution. Note: The final concentration of the Track It™ Orange working solution should be empirically determined for different cell types and/or experimental conditions. It is recommended to test at the concentrations that are at least over a ten-fold range.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Add 10X Track It™ Orange working solution to the cells, the volume should be equal to 1/10 of the volume of cell culture medium. For example, for a 96-well plate, add 10 µL/well of 10X Track It™ Orange working solution into the cells.
- Incubate the cells in a 37°C, 5% CO2 incubator for 15 minutes to 1 hour.
- Wash cells with Hanks and 20 mM Hepes buffer (HBSS) or an appropriate buffer.
- Fill the cell wells with growth medium.
- Analyze the cells using a fluorescence microscope with Cy3/TRITC filter sets of flow cytometer with 575/26 nm filter (PE channel).
Images
Citations
Authors: Sekine, Hidekazu and Homma, Jun and Shimizu, Tatsuya
Journal: (2022)
Authors: Zhang, Jun and Byers, Patrick and Erben, Amelie and Frank, Christine and Schulte-Spechtel, Levin and Heymann, Michael and Docheva, Denitsa and Huber, Heinz P and Sudhop, Stefanie and Clausen-Schaumann, Hauke
Journal: Advanced Functional Materials (2021): 2100066
Authors: Cemma, Marija and Grinstein, Sergio and Brumell, John H
Journal: Autophagy (2016): 1440--1446
Authors: Myung, Ja Hye and Gajjar, Khyati A and Chen, Jihua and Molokie, Robert E and Hong, Seungpyo
Journal: Analytical chemistry (2014): 6088--6094
Authors: Lei, Bo and Chen, Xiaofeng and Han, Xue and Zhou, Jiaan
Journal: Journal of Materials Chemistry (2012): 16906--16913
References
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Journal: Methods Mol Biol (2007): 41
Authors: Hoffman AF, Garippa RJ.
Journal: Methods Mol Biol (2007): 19
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Journal: Methods Mol Biol (2007): 189
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Journal: Methods Mol Biol (2007): 3
Authors: Martinez ED, Dull AB, Beutler JA, Hager GL.
Journal: Methods Enzymol (2006): 21
Authors: Bowen WP, Wylie PG.
Journal: Assay Drug Dev Technol (2006): 209
Authors: Hudson CC, Oakley RH, Sjaastad MD, Loomis CR.
Journal: Methods Enzymol (2006): 63
Authors: Werner T, Liebisch G, Gr and l M, Schmitz G.
Journal: Cytometry A (2006): 200
Authors: Wolff M, Haasen D, Merk S, Kroner M, Maier U, Bordel S, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
Journal: Comb Chem High Throughput Screen (2006): 339
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Application notes
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