LysoBrite™ Red
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Molecular weight | 698.94 |
Solvent | DMSO |
Excitation (nm) | 576 |
Emission (nm) | 596 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
LysoBrite™ Blue |
LysoBrite™ Green |
LysoBrite™ Orange |
LysoBrite™ Deep Red |
LysoBrite™ NIR |
LysoBrite™ Red DND-99 |
LysoBrite™ Green DND-26 |
Overview | SDSProtocol |
Molecular weight 698.94 | Excitation (nm) 576 | Emission (nm) 596 |
Platform
Flow cytometer
Excitation | 532/561 nm laser |
Emission | 585/40 nm filter |
Fluorescence microscope
Excitation | TRITC filter set |
Emission | TRITC filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Prepare cells.
Add dye working solution.
Incubate at 37 °C for 30 minutes.
Wash the cells.
Analyze under a fluorescence microscope.
The LysoBrite™ Red stock solution provided is 500X in DMSO. It should be stable for at least 6 months if stored at -20°C and protected from light. Avoid freeze/thaw cycles.
PREPARATION OF WORKING SOLUTION
Warm LysoBrite™ Red dye to room temperature.
Prepare dye working solution by diluting 20 µL of 500X LysoBrite™ Red with 10 mL of Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice.
Note: 20 µL of LysoBrite™ Red dye is enough for one 96-well plate. Aliquot and store unused LysoBrite™ dye stock solutions at < -15 °C. Protect it from light and avoid repeated freeze-thaw cycles.
Note: The optimal concentration of the fluorescent lysosome indicator varies depending on the specific application. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
SAMPLE EXPERIMENTAL PROTOCOL
This protocol only provides a guideline and should be modified according to your specific needs.
Grow cells in a 96-well black wall/clear bottom plate (100 µL/well/96-well plate) or on coverslips inside a petri dish filled with the appropriate culture medium.
When cells reach the desired confluence, add an equal volume of the dye-working solution (from Preparation of Working Solution Step 2).
Incubate the cells in a 37 °C, 5% CO2 incubator for 30 minutes.
Wash the cells twice with pre-warmed (37 °C) Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice. Then fill the cell wells with HBSS or growth medium.
Observe the cells using a fluorescence microscope fitted with the desired filter set.
Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
Add an equal volume of the dye-working solution (from Preparation of Working Solution Step 2).
Incubate the cells in a 37 °C, 5% CO2 incubator for 30 minutes.
Wash the cells twice with pre-warmed (37 °C) Hanks and 20 mM HEPES buffer (HBSS) or buffer of your choice. Then fill the cell wells with HBSS or growth medium.
Observe the cells using a fluorescence microscope fitted with the desired filter set.
Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
Note: Suspension cells may be attached to coverslips treated with BD Cell-Tak® (BD Biosciences) and stained as adherent cells (see Protocol for Preparing and Staining Adherent Cells).
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 143.074 µL | 715.369 µL | 1.431 mL | 7.154 mL | 14.307 mL |
5 mM | 28.615 µL | 143.074 µL | 286.148 µL | 1.431 mL | 2.861 mL |
10 mM | 14.307 µL | 71.537 µL | 143.074 µL | 715.369 µL | 1.431 mL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
LysoBrite™ Blue | 434 | 480 |
LysoBrite™ Green | 501 | 510 |
LysoBrite™ Orange | 543 | 565 |
LysoBrite™ Deep Red | 597 | 619 |
LysoBrite™ NIR | 636 | 651 |
Images
Citations
Authors: Zhang, Shunyao and Tamura, Atsushi and Yui, Nobuhiko
Journal: Biomolecules (2024): 223
Authors: Bao, Xingting and Liu, Xiongxiong and Wu, Qingfeng and Ye, Fei and Shi, Zheng and Xu, Dan and Zhang, Jinhua and Dou, Zhihui and Huang, Guomin and Zhang, Hong and others,
Journal: Antioxidants (2023): 453
Authors: Hirata, Yoko and Hashimoto, Tomohiro and Ando, Kaori and Kamatari, Yuji O and Takemori, Hiroshi and Furuta, Kyoji
Journal: RSC Advances (2023): 32276--32281
Authors: Hirata, Yoko and Oka, Kohei and Yamamoto, Shotaro and Watanabe, Hiroki and Oh-Hashi, Kentaro and Hirayama, Tasuku and Nagasawa, Hideko and Takemori, Hiroshi and Furuta, Kyoji
Journal: ACS Chemical Neuroscience (2022): 2719--2727
Authors: Kibel, Adam S and Inman, Brant A and Pachynski, Russell K and Vu, Tuyen and Sheikh, Nadeem A and Petrylak, Daniel P
Journal: JNCI: Journal of the National Cancer Institute (2021)
Authors: Fedotov, Sergei and Han, Daniel and Zubarev, Andrey Yu and Johnston, Mark and Allan, Victoria J
Journal: arXiv preprint arXiv:2101.02698 (2021)
Authors: Pettygrove, Brian A and Kratofil, Rachel M and Alhede, Maria and Jensen, Peter {\O} and Newton, Michelle and Qvortrup, Klaus and Pallister, Kyler B and Bjarnsholt, Thomas and Kubes, Paul and Voyich, Jovanka M and others,
Journal: Biomaterials (2021): 120775
Authors: Wang, Kun and Zhan, Yujuan and Chen, Bonan and Lu, Yuhua and Yin, Ting and Zhou, Shikun and Zhang, Weibin and Liu, Xiaodong and Du, Biaoyan and Wei, Xianli and others,
Journal: Cell death \& disease (2020): 1--16
Authors: Han, Daniel and Korabel, Nickolay and Chen, Runze and Johnston, Mark and Gavrilova, Anna and Allan, Victoria J and Fedotov, Sergei and Waigh, Thomas A
Journal: Elife (2020)
Authors: Xu, Gang and Ma, Xiujuan and Chen, Fang and Wu, Di and Miao, Jianing and Fan, Yang
Journal: Life Sciences (2020): 117532
References
Authors: Gough AH, Johnston PA.
Journal: Methods Mol Biol (2007): 41
Authors: Hoffman AF, Garippa RJ.
Journal: Methods Mol Biol (2007): 19
Authors: Giuliano KA., undefined
Journal: Methods Mol Biol (2007): 189
Authors: Taylor DL., undefined
Journal: Methods Mol Biol (2007): 3
Authors: Martinez ED, Dull AB, Beutler JA, Hager GL.
Journal: Methods Enzymol (2006): 21
Authors: Bowen WP, Wylie PG.
Journal: Assay Drug Dev Technol (2006): 209
Authors: Hudson CC, Oakley RH, Sjaastad MD, Loomis CR.
Journal: Methods Enzymol (2006): 63
Authors: Werner T, Liebisch G, Gr and l M, Schmitz G.
Journal: Cytometry A (2006): 200
Authors: Wolff M, Haasen D, Merk S, Kroner M, Maier U, Bordel S, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
Journal: Comb Chem High Throughput Screen (2006): 339
Authors: O'Brien P J, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C.
Journal: Arch Toxicol (2006): 580
Application notes
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?