Fluoro-Jade®B

Fluoro-Jade® B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It has a green fluorescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-Jade B is the FITC filter. Fluoro-Jade B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 um. Fluoro-Jade B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both gross and fine scale neuronal degeneration. Fluoro-Jade B will not distinguish between apoptotic and necrotic neurons. Astrocytic labeling is occasionally observed with Fluoro-Jade B, especially in chronic studies. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23061	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluoro-Jade®B to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	146.843 µL	734.214 µL	1.468 mL	7.342 mL	14.684 mL
5 mM	29.369 µL	146.843 µL	293.686 µL	1.468 mL	2.937 mL
10 mM	14.684 µL	73.421 µL	146.843 µL	734.214 µL	1.468 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	681 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Critical involvement of lysyl oxidase in seizure-induced neuronal damage through ERK-Alox5-dependent ferroptosis and its therapeutic implications

Authors:

Mao, Xiaoyuan and Wang, Xuan and Jin, Mingzhu and Li, Qin and Jia, Jining and Li, Menghuan and Zhou, Honghao and Liu, Zhaoqian and Jin, Weilin and Zhao, Yanli and others,

Journal:

Acta Pharmaceutica Sinica B (2022)

CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9

Authors:

Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang

Journal:

Cell Cycle (2021): 1828--1844

LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/$\beta$-catenin signaling pathway

Authors:

Jiang, Jingjing and Piao, Xuanyu and Hu, Siying and Gao, Jingbo and Bao, Min

Journal:

Aging (Albany NY) (2020): 8820

FTY720 attenuates behavioral deficits in a murine model of systemic lupus erythematosus

Authors:

Shi, Dongyan and Tian, Tongguan and Yao, Shu and Cao, Kelei and Zhu, Xingxing and Zhang, Mingshun and Wen, Shuang and Li, Longjun and Shi, Meiqing and Zhou, Hong

Journal:

Brain, behavior, and immunity (2018)

Early Minocycline and Late FK506 Treatment Improves Survival and Alleviates Neuroinflammation, Neurodegeneration, and Behavioral Deficits in Prion-Infected Hamsters

Authors:

Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Khan, Sher Hayat and Hussain, Tariq and Dong, Haodi and Lai, Mengyu and Zhou, Xiangmei and Yang, Lifeng

Journal:

Neurotherapeutics (2017): 1--21

References

View all 38 references: Citation Explorer

Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia

Authors:

Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.

Journal:

Cell Mol Neurobiol. (2013)

Neuronal damage using fluoro-jade B histofluorescence and gliosis in the striatum after various durations of transient cerebral ischemia in gerbils

Authors:

Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.

Journal:

Neurochem Res (2012): 826

Neuronal damage in hippocampal subregions induced by various durations of transient cerebral ischemia in gerbils using Fluoro-Jade B histofluorescence

Authors:

Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.

Journal:

Brain Res (2012): 50

One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C

Authors:

Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.

Journal:

J Neurosci Methods (2012): 40

Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest

Authors:

Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.

Journal:

Brain Res (2011): 127

Physical properties

Molecular weight	681
Solvent	Water

Spectral properties

Excitation (nm)	494
Emission (nm)	519

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on July 12, 2023