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Phenol Red, sodium salt *CAS 34487-61-6*

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Physical properties
Molecular weight376.36
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C); Minimize light exposure
UNSPSC12352200

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CAS
34487-61-6
Molecular weight
376.36
Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator that is frequently used in cell biology laboratories. Its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Above pH 8.1, phenol red turns a bright pink (fuschia) color. When cells are grown in tissue culture, the medium in which they grow is held close to the physiological pH. A small amount of phenol red added to the growth media will have a pink-red color under normal conditions. In the event of problems, waste products produced by dying cells or overgrowth of contaminants will cause a change in pH, leading to a change in indicator color.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Phenol Red, sodium salt *CAS 34487-61-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM265.703 µL1.329 mL2.657 mL13.285 mL26.57 mL
5 mM53.141 µL265.703 µL531.406 µL2.657 mL5.314 mL
10 mM26.57 µL132.852 µL265.703 µL1.329 mL2.657 mL

Molarity calculator

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Product Family


NameExcitation (nm)Emission (nm)
Calcein Red™ sodium salt562576

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References


View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132
Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702
Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938
Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208