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6-JOE alkyne

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Physical properties
Molecular weight542.32
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Alternative formats
6-JOE acid
6-JOE azide

OverviewpdfSDSpdfProtocol


Molecular weight
542.32
Extinction coefficient (cm -1 M -1)
750001
Excitation (nm)
520
Emission (nm)
545
6-JOE is widely used in nucleic acid sequencing and related research. 6-JOE alkyne provides a convenient method for labeling azide-modified oligos throught the well-known click chemistry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-JOE alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM184.393 µL921.965 µL1.844 mL9.22 mL18.439 mL
5 mM36.879 µL184.393 µL368.786 µL1.844 mL3.688 mL
10 mM18.439 µL92.196 µL184.393 µL921.965 µL1.844 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)750001
Excitation (nm)520
Emission (nm)545

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Alkyne493517830000.178
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*520545750001-
6-HEX alkyne533559730000.15
6-TET alkyne521542730000.13
6-TAMRA alkyne552578900000.178
6-ROX alkyne578604820000.168
6-JOE Phosphoramidite520545750001-
6-NED alkyne545567740001-
6-VIC Alkyne526543--

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References


View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058