5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*
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Physical properties
Molecular weight | 430.45 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
See also: Rhodamines and Rhodamine Derivatives, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR)
CAS 98181-63-6 | Molecular weight 430.45 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.178 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 552 | Emission (nm) 578 |
TAMRA is one of the most popular fluorophores used in various bioconjugations. TMR is a bright orange fluorophore. 5(6)-TAMRA is the mixture of two carboxy tetramethylrhodamine (TMR) isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings. It can be readily convert to the amine-reactive 5(6)-TAMRA, SE.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 232.315 µL | 1.162 mL | 2.323 mL | 11.616 mL | 23.232 mL |
5 mM | 46.463 µL | 232.315 µL | 464.63 µL | 2.323 mL | 4.646 mL |
10 mM | 23.232 µL | 116.158 µL | 232.315 µL | 1.162 mL | 2.323 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.178 |
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 552 |
Emission (nm) | 578 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G] | 522 | 546 | 94000 | 0.24 | 0.214 |
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7* | 552 | 578 | 90000 | 0.32 | 0.178 |
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* | 552 | 578 | 90000 | 0.32 | 0.178 |
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8* | 578 | 604 | 82000 | - | 0.168 |
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8* | 544 | 570 | 100000 | 0.27 | 0.34 |
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5* | 552 | 578 | 90000 | 0.32 | 0.178 |
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-TAMRA cadaverine | 552 | 578 | 90000 | 0.32 | 0.178 |
Show More (8) |
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Citations
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Journal: Organic \& Biomolecular Chemistry (2020): 1978--1986
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Journal: Frontiers in microbiology (2018)
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Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
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Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
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Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
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References
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A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
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Authors: Nakayama GR, Bingham P, Tan D, Maegley KA.
Journal: Anal Biochem (2006): 260
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
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Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
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Authors: Fischer R, Bachle D, Fotin-Mleczek M, Jung G, Kalbacher H, Brock R.
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Authors: Juskowiak B, Galezowska E, Zawadzka A, Gluszynska A, Takenaka S.
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High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
Authors: Roman GT, McDaniel K, Culbertson CT.
Journal: Analyst (2006): 194
Authors: Roman GT, McDaniel K, Culbertson CT.
Journal: Analyst (2006): 194
Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8
Authors: Maruyama T, Shinohara T, Hosogi T, Ichinose H, Kamiya N, Goto M.
Journal: Anal Biochem (2006): 8
Optical detection of DNA hybridization based on fluorescence quenching of tagged oligonucleotide probes by gold nanoparticles
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Authors: Wu ZS, Jiang JH, Fu L, Shen GL, Yu RQ.
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Fluorescence quenching by TEMPO: a sub-30 A single-molecule ruler
Authors: Zhu P, Clamme JP, Deniz AA.
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Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
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FAQ
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