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6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*

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Physical properties
Molecular weight527.53
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Show More (42)

OverviewpdfSDSpdfProtocol


CAS
150810-69-8
Molecular weight
527.53
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.178
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)
552
Emission (nm)
578
The single TAMRA isomers are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purification that is often required in the conjugation processes. 6-TAMRA, SE is predominantly used for labeling nucleotides and nucleic acids.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.563 µL947.813 µL1.896 mL9.478 mL18.956 mL
5 mM37.913 µL189.563 µL379.125 µL1.896 mL3.791 mL
10 mM18.956 µL94.781 µL189.563 µL947.813 µL1.896 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)90000
Excitation (nm)552
Emission (nm)578

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Citations


View all 4 citations: Citation Explorer
Thiol-ene conjugation of VEGF peptide to electrospun scaffolds as potential application for angiogenesis
Authors: Yao, Tianyu and Chen, Honglin and Wang, Rong and Rivero, Rebeca and Wang, Fengyu and Kessels, Lilian and Agten, Stijn M and Hackeng, Tilman M and Wolfs, Tim GAM and Fan, Daidi and others,
Journal: Bioactive Materials (2023): 306--317
Design and Characterization of Two Bifunctional Cryptophane A-Based Host Molecules for Xenon Magnetic Resonance Imaging Applications
Authors: Rossella, Federica and Rose, Honor May and Witte, Christopher and Jayapaul, Jabadurai and Schr&ouml;der, Leif
Journal: ChemPlusChem (2014): 1463--1471
Human erythrocytes as drug carriers: loading efficiency and side effects of hypotonic dialysis, chlorpromazine treatment and fusion with liposomes
Authors: Favretto, ME and Cluitmans, JCA and Bosman, GJCGM and Brock, R
Journal: Journal of Controlled Release (2013): 343--351
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, S&oslash;ren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

References


View all 96 references: Citation Explorer
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A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase
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High efficiency micellar electrokinetic chromatography of hydrophobic analytes on poly(dimethylsiloxane) microchips
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Metal-enhanced fluorescence-based RNA sensing
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Fluorescence anisotropy and FRET studies of G-quadruplex formation in presence of different cations
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Electrostatic-gated transport in chemically modified glass nanopore electrodes
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A targeted protease substrate for a quantitative determination of protease activities in the endolysosomal pathway
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Journal: Chembiochem (2006): 1428
G Quadruplex-Based FRET Probes with the Thrombin-Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion
Authors: Nagatoishi S, Nojima T, Galezowska E, Juskowiak B, Takenaka S.
Journal: Chembiochem (2006): 1730
Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
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Journal: Photochem Photobiol (2005): 682