logo
AAT Bioquest

6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight534.60
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Related products
Rhodamine 700 *CAS 63561-42-2*
Rhodamine 800 *CAS 137993-41-0*
Rhodamine B *CAS 81-88-9*
Rhodamine 6G *CAS 989-38-8*
Sulforhodamine 101, sodium salt
Sulforhodamine B *CAS 3520-42-1*
Sulforhodamine G *CAS 5873-16-5*
Rhodamine 101 Inner Salt *CAS 116450-56-7*
Rhodamine 101 Chloride Salt *CAS 64339-18-0*
Sulforhodamine 101 sodium salt *CAS 465544-24-5*
Rhodamine 110 *CAS 13558-31-1*
5-CR110 [5-Carboxyrhodamine 110] *Single isomer*
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5(6)-Carboxyrhodamine 6G cadaverine
5(6)-Carboxyrhodamine 6G ethylenediamine
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4*
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4*
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*
Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*
Sulforhodamine 101 sulfonyl chloride [Texas Red®] *CAS 82354-19-6*
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]
Dihydrorhodamine 123 *CAS 109244-58-8*
Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*
Rhodamine 123 *CAS 62669-70-9*
Rhodamine B, hexyl ester, perchlorate
TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
Phalloidin-Tetramethylrhodamine Conjugate
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
Bis-Arg-rhodamine 110
Sulforhodamine 101 *CAS 60311-02-6*
5-Carboxyrhodamine 6G maleimide
Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (40)

OverviewpdfSDSpdfProtocol


CAS
194785-18-7
Molecular weight
534.60
Correction Factor (280 nm)
0.168
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
578
Emission (nm)
604
Quantum yield
0.941
6-ROX is the other purified single isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. 6-ROX is predominantly used to label nucleotides and for DNA sequencing. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 6-ROX without the conventional chromatographic purification. Cautions must be exercised to prevent the deterioration during storage.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM187.056 µL935.279 µL1.871 mL9.353 mL18.706 mL
5 mM37.411 µL187.056 µL374.111 µL1.871 mL3.741 mL
10 mM18.706 µL93.528 µL187.056 µL935.279 µL1.871 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (280 nm)0.168
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)578
Emission (nm)604
Quantum yield0.941

Images


References


View all 11 references: Citation Explorer
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2005): 5926
Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: Langmuir (2004): 6834
Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: Proc Natl Acad Sci U S A (2004): 5488
Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: Faulds K, Smith WE, Graham D.
Journal: Anal Chem (2004): 412
Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: Invest Ophthalmol Vis Sci (2003): 1312
HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: Tissue Antigens (2001): 250
Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis
Authors: Hahn M, Wilhelm J, Pingoud A.
Journal: Electrophoresis (2001): 2691
Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: Li Y, Glazer AN.
Journal: Bioconjug Chem (1999): 241
Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: Anal Biochem (1998): 82
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: Hung SC, Mathies RA, Glazer AN.
Journal: Anal Biochem (1998): 32