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FMOC-Asp(EDANS)-OH *CAS 182253-73-2*

FMOC-Asp(EDANS)-OH is a building block for in-sequence peptide Asp labeling by EDANS. EDANS is one of the most common donor dyes for preparing FRET peptides (often paired with Dabcyl).

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(EDANS)-OH *CAS 182253-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM165.662 µL828.308 µL1.657 mL8.283 mL16.566 mL
5 mM33.132 µL165.662 µL331.323 µL1.657 mL3.313 mL
10 mM16.566 µL82.831 µL165.662 µL828.308 µL1.657 mL

Molarity calculator

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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)
FMOC-Asp(TF3)-OH7500010.179
FMOC-Glu(EDANS)-OH *CAS 193475-66-0*59000.107
FMOC-Asp(DABCYL)-OH-0.516
FMOC-Asp(TQ2)-OH210000.12
FMOC-Asp(TQ3)-OH220000.091

Citations

View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Page updated on September 18, 2024

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Physical properties

Molecular weight

603.64

Solvent

DMF

Spectral properties

Correction Factor (280 nm)

0.107

Extinction coefficient (cm -1 M -1)

5900

Excitation (nm)

336

Emission (nm)

455

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

182253-73-2
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