Calculator
AMCA Alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 270.28 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.153 |
| Extinction coefficient (cm -1 M -1) | 19000 |
| Excitation (nm) | 346 |
| Emission (nm) | 434 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| AMCA, succinimidyl ester |
| AMCA Azide |
| Overview |  SDSProtocol |
See also: Coumarins, Click Chemistry
Molecular weight 270.28 | Correction Factor (280 nm) 0.153 | Extinction coefficient (cm -1 M -1) 19000 | Excitation (nm) 346 | Emission (nm) 434 |
AMCA is one of the brightest blue fluorescent labeling dyes. It can be well excited at 365 nm. It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. This AMC dye can be used for labeling alkyne compounds through click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of AMCA Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 369.987 µL | 1.85 mL | 3.7 mL | 18.499 mL | 36.999 mL |
| 5 mM | 73.997 µL | 369.987 µL | 739.973 µL | 3.7 mL | 7.4 mL |
| 10 mM | 36.999 µL | 184.993 µL | 369.987 µL | 1.85 mL | 3.7 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.153 |
| Extinction coefficient (cm -1 M -1) | 19000 |
| Excitation (nm) | 346 |
| Emission (nm) | 434 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | 0.153 |
| AMCA Ethylenediamine | 346 | 434 | 19000 | 0.153 |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| ICG alkyne | 789 | 813 | 230000 | 0.076 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
Images
Figure 1. Chemical structure for AMCA Alkyne
References
View all 13 references: Citation Explorer
Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1
Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160
Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518
Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240
Oral surgical procedures during anticoagulant therapy
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681
Soluble aggregates of IgG and immune complexes enhance IL-6 production by renal mesangial cells
Authors: van den Dobbelsteen ME, van der Woude FJ, Schroeijers WE, van Es LA, Daha MR.
Journal: Kidney Int (1993): 544
Authors: van den Dobbelsteen ME, van der Woude FJ, Schroeijers WE, van Es LA, Daha MR.
Journal: Kidney Int (1993): 544
Evidence for cobinding of self- and allopeptides to human class II major histocompatibility antigen DR1 by energy transfer
Authors: Kropshofer H, Max H, Kalbacher H.
Journal: Proc Natl Acad Sci U S A (1993): 403
Authors: Kropshofer H, Max H, Kalbacher H.
Journal: Proc Natl Acad Sci U S A (1993): 403
Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide
Authors: Eis PS, Lakowicz JR.
Journal: Biochemistry (1993): 7981
Authors: Eis PS, Lakowicz JR.
Journal: Biochemistry (1993): 7981
Improved indirect fluorescence immunocytochemical method using counterstains
Authors: Newkirk RF, Mack J.
Journal: Biotechniques (1992): 536
Authors: Newkirk RF, Mack J.
Journal: Biotechniques (1992): 536
Triple immunofluorescence confocal laser scanning microscopy: spatial correlation of novel cellular differentiation markers in human muscle biopsies
Authors: Schubert W., undefined
Journal: Eur J Cell Biol (1991): 272
Authors: Schubert W., undefined
Journal: Eur J Cell Biol (1991): 272