FMOC-Lys(TQ2)-OH
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 732.89 |
Solvent | DMF |
Spectral properties
Absorbance (nm) | 516 |
Correction Factor (260 nm) | 0.100 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 21000 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Molecular weight 732.89 | Absorbance (nm) 516 | Correction Factor (260 nm) 0.100 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 21000 |
FMOC-Lys(TQ2)-OH is a building block for in-sequence Lys labeling by Tide Quencher™ 2 (TQ2). TQ2 is the best quencher that is used to develop a FRET probe with fluorescein or other fluorescent dyes that have the similar fluorescence spectra (to fluoresceins) such as Bodipy FL dyes. It can also be used with rhodamine 6G, 6-TET and 6-JOE derivatives.
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(TQ2)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 136.446 µL | 682.231 µL | 1.364 mL | 6.822 mL | 13.645 mL |
5 mM | 27.289 µL | 136.446 µL | 272.892 µL | 1.364 mL | 2.729 mL |
10 mM | 13.645 µL | 68.223 µL | 136.446 µL | 682.231 µL | 1.364 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 516 |
Correction Factor (260 nm) | 0.100 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 21000 |
Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) | Correction Factor (260 nm) |
FMOC-Lys(DABCYL)-OH *CAS 146998-27-8* | - | 0.516 | - |
FMOC-Lys(TF3)-OH | 750001 | 0.179 | - |
FMOC-Lys(Cy3)-OH | 1500001 | 0.073 | 0.07 |
FMOC-Lys(Cy5)-OH | 2500001 | 0.03 | 0.02 |
FMOC-Asp(TQ2)-OH | 21000 | 0.12 | 0.100 |
FMOC-Glu(TQ2)-OH | 21000 | 0.12 | 0.100 |
FMOC-Lys(TQ3)-OH | 22000 | 0.091 | 0.085 |
Citations
View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
References
View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689
Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308
Controlled peptide solvation in portion-mixing libraries of FRET peptides: improved specificity determination for Dengue 2 virus NS2B-NS3 protease and human cathepsin S
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627
Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209
Application notes
Tide Quencher™-Based FRET Protease Substrates and Their Applications In Drug Discovery and Disease Diagnosis
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger
Bio-inspired NADH regeneration by carbon nitride photocatalysis using diatom templates
Restriction of Advanced Glycation End Products Improves Insulin Resistance in Human Type 2 Diabetes
Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
Inhibition of oxidative phosphorylation for enhancing citric acid production by Aspergillus niger
Bio-inspired NADH regeneration by carbon nitride photocatalysis using diatom templates