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FMOC-Lys(TQ2)-OH

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Physical properties
Molecular weight732.89
SolventDMF
Spectral properties
Absorbance (nm)516
Correction Factor (260 nm)0.100
Correction Factor (280 nm)0.12
Extinction coefficient (cm -1 M -1)21000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
732.89
Absorbance (nm)
516
Correction Factor (260 nm)
0.100
Correction Factor (280 nm)
0.12
Extinction coefficient (cm -1 M -1)
21000
FMOC-Lys(TQ2)-OH is a building block for in-sequence Lys labeling by Tide Quencher™ 2 (TQ2). TQ2 is the best quencher that is used to develop a FRET probe with fluorescein or other fluorescent dyes that have the similar fluorescence spectra (to fluoresceins) such as Bodipy FL dyes. It can also be used with rhodamine 6G, 6-TET and 6-JOE derivatives.

Calculators


Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(TQ2)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM136.446 µL682.231 µL1.364 mL6.822 mL13.645 mL
5 mM27.289 µL136.446 µL272.892 µL1.364 mL2.729 mL
10 mM13.645 µL68.223 µL136.446 µL682.231 µL1.364 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)516
Correction Factor (260 nm)0.100
Correction Factor (280 nm)0.12
Extinction coefficient (cm -1 M -1)21000

Product Family


NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)Correction Factor (260 nm)
FMOC-Lys(DABCYL)-OH *CAS 146998-27-8*-0.516-
FMOC-Lys(TF3)-OH7500010.179-
FMOC-Lys(Cy3)-OH15000010.0730.07
FMOC-Lys(Cy5)-OH25000010.030.02
FMOC-Asp(TQ2)-OH210000.120.100
FMOC-Glu(TQ2)-OH210000.120.100
FMOC-Lys(TQ3)-OH220000.0910.085

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Citations


View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References


View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65
Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689
Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308
Controlled peptide solvation in portion-mixing libraries of FRET peptides: improved specificity determination for Dengue 2 virus NS2B-NS3 protease and human cathepsin S
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627
Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209