7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*
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Physical properties
Molecular weight | 206.15 |
Solvent | DMSO |
Spectral properties
Correction Factor (280 nm) | 0.0815 |
Excitation (nm) | 352 |
Emission (nm) | 407 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Coumarins
CAS 779-27-1 | Molecular weight 206.15 | Correction Factor (280 nm) 0.0815 | Excitation (nm) 352 | Emission (nm) 407 |
7-Hydroxycoumarin-3-carboxylic acid is a blue fluorophores for labeling proteins and nucleic acids mostly through the in situ formation of its succinimidyl ester catalyzed by EDAC.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 485.084 µL | 2.425 mL | 4.851 mL | 24.254 mL | 48.508 mL |
5 mM | 97.017 µL | 485.084 µL | 970.167 µL | 4.851 mL | 9.702 mL |
10 mM | 48.508 µL | 242.542 µL | 485.084 µL | 2.425 mL | 4.851 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.0815 |
Excitation (nm) | 352 |
Emission (nm) | 407 |
Product Family
Name | Excitation (nm) | Emission (nm) | Correction Factor (280 nm) |
7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* | 355 | 405 | 0.171 |
References
View all 16 references: Citation Explorer
In vitro anti-tumour and cyto-selective effects of coumarin-3-carboxylic acid and three of its hydroxylated derivatives, along with their silver-based complexes, using human epithelial carcinoma cell lines
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Authors: Thati B, Noble A, Creaven BS, Walsh M, McCann M, Kavanagh K, Devereux M, Egan DA.
Journal: Cancer Lett. (2006)
Effects of iron on Vitamin C/copper-induced hydroxyl radical generation in bicarbonate-rich water
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Authors: Jansson PJ, Del Castillo U, Lindqvist C, Nordstrom T.
Journal: Free Radic Res (2005): 565
Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
Authors: Hashidoko Y, Tanaka T, Tahara S.
Journal: Biosci Biotechnol Biochem (2001): 2604
Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III)
Authors: Chakrabarti S, Mahmood A, Makrigiorgos GM.
Journal: Int J Radiat Biol (1999): 1055
Authors: Chakrabarti S, Mahmood A, Makrigiorgos GM.
Journal: Int J Radiat Biol (1999): 1055
Flow injection analysis of binding reaction between fluorescent lectin and cells
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
Authors: Oda Y, Kinoshita M, Nakayama K, Ikeda S, Kakehi K.
Journal: Anal Biochem (1999): 230
A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Authors: Higai K, Masuda D, Matsuzawa Y, Satoh T, Matsumoto K.
Journal: Biol Pharm Bull (1999): 333
Autoxidation of ferrous ion complexes: a method for the generation of hydroxyl radicals
Authors: Kachur AV, Tuttle SW, Biaglow JE.
Journal: Radiat Res (1998): 475
Authors: Kachur AV, Tuttle SW, Biaglow JE.
Journal: Radiat Res (1998): 475
Trans/cis (Z/E) photoisomerization of the chromophore of photoactive yellow protein is not a prerequisite for the initiation of the photocycle of this photoreceptor protein
Authors: Cordfunke R, Kort R, Pierik A, Gobets B, Koomen GJ, Verhoeven JW, Hellingwerf KJ.
Journal: Proc Natl Acad Sci U S A (1998): 7396
Authors: Cordfunke R, Kort R, Pierik A, Gobets B, Koomen GJ, Verhoeven JW, Hellingwerf KJ.
Journal: Proc Natl Acad Sci U S A (1998): 7396
Coumarin-3-carboxylic acid as a detector for hydroxyl radicals generated chemically and by gamma radiation
Authors: Manevich Y, Held KD, Biaglow JE.
Journal: Radiat Res (1997): 580
Authors: Manevich Y, Held KD, Biaglow JE.
Journal: Radiat Res (1997): 580
Effect of purine nucleoside phosphates on OH-radical generation by reaction of Fe2+ with oxygen
Authors: Kachur AV, Manevich Y, Biaglow JE.
Journal: Free Radic Res (1997): 399
Authors: Kachur AV, Manevich Y, Biaglow JE.
Journal: Free Radic Res (1997): 399
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