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7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8*

7-Methoxycoumarin-3-carboxylic acid is used for HPLC derivatization. It is also used to develop FRET probes for analyzing protease activities.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 7-Methoxycoumarin-3-carboxylic acid *CAS 20300-59-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM454.174 µL2.271 mL4.542 mL22.709 mL45.417 mL
5 mM90.835 µL454.174 µL908.348 µL4.542 mL9.083 mL
10 mM45.417 µL227.087 µL454.174 µL2.271 mL4.542 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Correction Factor (280 nm)
7-Hydroxycoumarin-3-carboxylic acid *CAS 779-27-1*3524070.0815

References

View all 3 references: Citation Explorer
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence
Authors: Berthelot T, Talbot JC, Lain G, Deleris G, Latxague L.
Journal: J Pept Sci (2005): 153
An alternative quenched fluorescence substrate for Pz-peptidase
Authors: Tisljar U, et al.
Journal: Anal Biochem (1990): 112-5
Quantitation of platelet-activating factor by high-performance liquid chromatography with fluorescent detection
Authors: Mita H, Yasueda H, Hayakawa T, Shida T.
Journal: Anal Biochem (1989): 131
Page updated on September 19, 2024

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Physical properties

Molecular weight

220.18

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.171

Excitation (nm)

355

Emission (nm)

405

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

20300-59-8
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