Pyronin Y *CAS 92-32-0*
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Physical properties
Molecular weight | 302.8 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 550 |
Excitation (nm) | 547 |
Emission (nm) | 566 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Nucleus
CAS 92-32-0 | Molecular weight 302.8 | Absorbance (nm) 550 | Excitation (nm) 547 | Emission (nm) 566 |
Pyronin Y is widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Pyronin Y *CAS 92-32-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 330.251 µL | 1.651 mL | 3.303 mL | 16.513 mL | 33.025 mL |
5 mM | 66.05 µL | 330.251 µL | 660.502 µL | 3.303 mL | 6.605 mL |
10 mM | 33.025 µL | 165.125 µL | 330.251 µL | 1.651 mL | 3.303 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 550 |
Excitation (nm) | 547 |
Emission (nm) | 566 |
Citations
View all 1 citations: Citation Explorer
Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957
References
View all 34 references: Citation Explorer
A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27
Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299
Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193
A methyl green-pyronin technique for demonstrating cell death in the murine tumour S180
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677
Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132
Dye standards, Part II. 1: Pyronin Y (CI 45005)
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220
Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305
1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209
Application notes
FAQ
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Are mRNAs found in prokaryotes differ from those of eukaryotes?
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Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?