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EDANS C2 maleimide

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Physical properties
Molecular weight346.35
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
2149639-01-8
Molecular weight
346.35
Correction Factor (280 nm)
0.107
Extinction coefficient (cm -1 M -1)
5900
Excitation (nm)
336
Emission (nm)
455
EDANS C2 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS C2 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM288.725 µL1.444 mL2.887 mL14.436 mL28.873 mL
5 mM57.745 µL288.725 µL577.451 µL2.887 mL5.775 mL
10 mM28.873 µL144.363 µL288.725 µL1.444 mL2.887 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
AMCA C2 Maleimide346434190000.153
DABCYL C2 maleimide---0.516
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*6506712390000.03
EDANS C5 maleimide33645559000.107

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References


View all 54 references: Citation Explorer
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Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
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Journal: Protein Expr Purif (2006): 88
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A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
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Characterization and inhibition of SARS-coronavirus main protease
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Journal: Curr Top Med Chem (2006): 361
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On the sequential determinants of calpain cleavage
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