DDAO [7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)] *CAS 118290-05-4*
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Physical properties
Molecular weight | 308.16 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 400001 |
Excitation (nm) | 648 |
Emission (nm) | 658 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
CAS 118290-05-4 | Molecular weight 308.16 | Extinction coefficient (cm -1 M -1) 400001 | Excitation (nm) 648 | Emission (nm) 658 |
DDAO [7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)] is used as a calibration standard for DDAO-based enzyme substrates. It has pH-dependent red fluorescence with excitation close to the He-Ne red laser line 633 nm.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DDAO [7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)] *CAS 118290-05-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 324.507 µL | 1.623 mL | 3.245 mL | 16.225 mL | 32.451 mL |
5 mM | 64.901 µL | 324.507 µL | 649.013 µL | 3.245 mL | 6.49 mL |
10 mM | 32.451 µL | 162.253 µL | 324.507 µL | 1.623 mL | 3.245 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 400001 |
Excitation (nm) | 648 |
Emission (nm) | 658 |
Images
Citations
View all 3 citations: Citation Explorer
Efficient Biofilm-Based Fermentation Strategies by eDNA Formation for l-Proline Production with Corynebacterium glutamicum
Authors: Ren, Peifang and Chen, Tianpeng and Liu, Na and Sun, Wenjun and Hu, Guang and Yu, Ying and Yu, Bin and Ouyang, Pingkai and Liu, Dong and Chen, Yong
Journal: ACS Omega (2020)
Authors: Ren, Peifang and Chen, Tianpeng and Liu, Na and Sun, Wenjun and Hu, Guang and Yu, Ying and Yu, Bin and Ouyang, Pingkai and Liu, Dong and Chen, Yong
Journal: ACS Omega (2020)
Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care
Authors: Schmalenberg, Michael and Beaudoin, Christopher and Bulst, Ludwig and Steubl, Dominik and Luppa, Peter B
Journal: Journal of immunological methods (2015): 36--41
Authors: Schmalenberg, Michael and Beaudoin, Christopher and Bulst, Ludwig and Steubl, Dominik and Luppa, Peter B
Journal: Journal of immunological methods (2015): 36--41
Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care
Authors: Schmalenberg, Michael and Beaudoin, Christopher and Bulst, Ludwig and Steubl, Dominik and Luppa, Peter B
Journal: Journal of Immunological Methods (2015): 36--41
Authors: Schmalenberg, Michael and Beaudoin, Christopher and Bulst, Ludwig and Steubl, Dominik and Luppa, Peter B
Journal: Journal of Immunological Methods (2015): 36--41
References
View all 5 references: Citation Explorer
Asymmetric fluorogenic organophosphates for the development of active organophosphate hydrolases with reversed stereoselectivity
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
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Journal: Toxicology (2007): 187
In vivo imaging of beta-galactosidase activity using far red fluorescent switch
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Journal: Cancer Res (2004): 1579
Authors: Tung CH, Zeng Q, Shah K, Kim DE, Schellingerhout D, Weissleder R.
Journal: Cancer Res (2004): 1579
Quantification of beta-galactosidase activity after non-viral transfection in vivo
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Journal: J Control Release (2003): 201
Authors: Zinselmeyer BH, Beggbie N, Uchegbu IF, Schatzlein AG.
Journal: J Control Release (2003): 201
Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates
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Journal: Proteomics (2003): 1215
Authors: Martin K, Hart C, Liu J, Leung WY, Patton WF.
Journal: Proteomics (2003): 1215
Characterization of 9H-(1,3-dichlor-9, 9-dimethylacridin-2-ona-7-yl)-phosphate (DDAO) as substrate of PP-2A in a fluorimetric microplate assay for diarrhetic shellfish toxins (DSP)
Authors: Leira F, Vieites JM, Vieytes MR, Botana LM.
Journal: Toxicon (2000): 1833
Authors: Leira F, Vieites JM, Vieytes MR, Botana LM.
Journal: Toxicon (2000): 1833