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APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 523.40 |
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 17160 |
| Excitation (nm) | 427 |
| Emission (nm) | 478 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
CAS 196504-57-1 | Molecular weight 523.40 | Extinction coefficient (cm -1 M -1) 17160 | Excitation (nm) 427 | Emission (nm) 478 |
This water-soluble reagent has strong green fluorescence with great Stokes shift. It is widely used for detecting carbohydrates and labeling glycoproteins in electrophoresis. It is also used to modify other carbonyl-containing biomolecules such as aldehydes and ketones.
Platform
Fluorescence microplate reader
| Excitation | 424 nm |
| Emission | 505 nm |
| Cutoff | 450 nm |
| Recommended plate | Solid black |
Example protocol
AT A GLANCE
Important notes
It is important to store APTS at <-15 °C and should be stored in cool, dark place.
It can be used within 24 months from the receiving date.
PREPARATION OF WORKING SOLUTION
APTS working solution:
Add water to make APTS working solution of desired concentration.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 191.058 µL | 955.292 µL | 1.911 mL | 9.553 mL | 19.106 mL |
| 5 mM | 38.212 µL | 191.058 µL | 382.117 µL | 1.911 mL | 3.821 mL |
| 10 mM | 19.106 µL | 95.529 µL | 191.058 µL | 955.292 µL | 1.911 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 17160 |
| Excitation (nm) | 427 |
| Emission (nm) | 478 |
Images
Figure 1. Chemical structure for APTS [8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt] *CAS 196504-57-1*
Citations
View all 2 citations: Citation Explorer
A rapid and facile preparation of APTS-labeled N-glycans by combination of ion pair-assisted extraction and HILIC-SPE for routine glycan analysis
Authors: Kinoshita, Mitsuhiro and Nakatani, Yumi and Yamada, Keita and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Journal of Pharmaceutical and Biomedical Analysis (2021): 113875
Authors: Kinoshita, Mitsuhiro and Nakatani, Yumi and Yamada, Keita and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Journal of Pharmaceutical and Biomedical Analysis (2021): 113875
High-throughput N-glycan screening method for therapeutic antibodies using a microchip-based DNA analyzer: a promising methodology for monitoring monoclonal antibody N-glycosylation
Authors: Kinoshita, Mitsuhiro and Nakajima, Kazuki and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Analytical and Bioanalytical Chemistry (2021): 1--12
Authors: Kinoshita, Mitsuhiro and Nakajima, Kazuki and Yamamoto, Sachio and Suzuki, Shigeo
Journal: Analytical and Bioanalytical Chemistry (2021): 1--12
References
View all 28 references: Citation Explorer
A novel tool to characterize paracellular transport: the APTS-dextran ladder
Authors: Neuhaus W, Bogner E, Wirth M, Trzeciak J, Lachmann B, Gabor F, Noe CR.
Journal: Pharm Res (2006): 1491
Authors: Neuhaus W, Bogner E, Wirth M, Trzeciak J, Lachmann B, Gabor F, Noe CR.
Journal: Pharm Res (2006): 1491
Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides
Authors: Kabel MA, Heijnis WH, Bakx EJ, Kuijpers R, Voragen AG, Schols HA.
Journal: J Chromatogr A. (2006)
Authors: Kabel MA, Heijnis WH, Bakx EJ, Kuijpers R, Voragen AG, Schols HA.
Journal: J Chromatogr A. (2006)
A novel ultra-sensitive method for the quantification of glycosaminoglycan disaccharides using an automated DNA sequencer
Authors: Vogel K, Kuhn J, Kleesiek K, Gotting C.
Journal: Electrophoresis (2006): 1363
Authors: Vogel K, Kuhn J, Kleesiek K, Gotting C.
Journal: Electrophoresis (2006): 1363
Capillary electrophoresis-mass spectrometry as a characterization tool for therapeutic proteins
Authors: Gennaro LA, Salas-Solano O, Ma S.
Journal: Anal Biochem (2006): 249
Authors: Gennaro LA, Salas-Solano O, Ma S.
Journal: Anal Biochem (2006): 249
APTS-labeled dextran ladder: a novel tool to characterize cell layer tightness
Authors: Neuhaus W, Trzeciak J, Lauer R, Lachmann B, Noe CR.
Journal: J Pharm Biomed Anal (2006): 1035
Authors: Neuhaus W, Trzeciak J, Lauer R, Lachmann B, Noe CR.
Journal: J Pharm Biomed Anal (2006): 1035
Capillary electrophoresis separation of a mixture of chitin and chitosan oligosaccharides derivatized using a modified fluorophore conjugation procedure
Authors: Beaudoin ME, Gauthier J, Boucher I, Waldron KC.
Journal: J Sep Sci (2005): 1390
Authors: Beaudoin ME, Gauthier J, Boucher I, Waldron KC.
Journal: J Sep Sci (2005): 1390
Total serum protein N-glycome profiling on a capillary electrophoresis-microfluidics platform
Authors: Callewaert N, Contreras R, Mitnik-Gankin L, Carey L, Matsudaira P, Ehrlich D.
Journal: Electrophoresis (2004): 3128
Authors: Callewaert N, Contreras R, Mitnik-Gankin L, Carey L, Matsudaira P, Ehrlich D.
Journal: Electrophoresis (2004): 3128
Capillary electrophoresis analysis of glucooligosaccharide regioisomers
Authors: Joucla G, Br and o T, Remaud-Simeon M, Monsan P, Puzo G.
Journal: Electrophoresis (2004): 861
Authors: Joucla G, Br and o T, Remaud-Simeon M, Monsan P, Puzo G.
Journal: Electrophoresis (2004): 861
Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs
Authors: Nakajima K, Kinoshita M, Oda Y, Masuko T, Kaku H, Shibuya N, Kakehi K.
Journal: Glycobiology (2004): 793
Authors: Nakajima K, Kinoshita M, Oda Y, Masuko T, Kaku H, Shibuya N, Kakehi K.
Journal: Glycobiology (2004): 793
Analysis of high-mannose-type oligosaccharides by microliquid chromatography-mass spectrometry and capillary electrophoresis
Authors: Koller A, Kh and urina J, Li J, Kreps J, Schieltz D, Guttman A.
Journal: Electrophoresis (2004): 2003
Authors: Koller A, Kh and urina J, Li J, Kreps J, Schieltz D, Guttman A.
Journal: Electrophoresis (2004): 2003