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mBBr [Monobromobimane] *CAS 71418-44-5*

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Physical properties
Molecular weight271.11
SolventDMSO
Spectral properties
Excitation (nm)395
Emission (nm)490
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
71418-44-5
Molecular weight
271.11
Excitation (nm)
395
Emission (nm)
490
mBBr is one of the most popular thiol-reactive fluorescent tags, and is widely used to detect various thiol-containing biomolecules such as glutathione in cells. It is fluorogenic upon reacting with thiol-containing molecules. mBBr is used for the determination of the redox status of low molecular weight and protein thiols in biological systems. MBBr-based in situ derivatization results in maximal recovery of both free, reduced low molecular weight and bromobimane accessible protein thiols. The quantitation of the corresponding adducts of protein thiols is achieved by fluorescence spectroscopy following protein precipitation.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of mBBr [Monobromobimane] *CAS 71418-44-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM368.854 µL1.844 mL3.689 mL18.443 mL36.885 mL
5 mM73.771 µL368.854 µL737.708 µL3.689 mL7.377 mL
10 mM36.885 µL184.427 µL368.854 µL1.844 mL3.689 mL

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Spectrum


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spectrum

Spectral properties

Excitation (nm)395
Emission (nm)490

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Citations


View all 1 citations: Citation Explorer
The development of antioxidant system in the intestinal tract of broiler chickens
Authors: Tang, Dazhi and Wu, Jianmin and Jiao, Hongchao and Wang, Xiaojuan and Zhao, Jingpeng and Lin, Hai
Journal: Poultry science (2019): 664--678

References


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