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Resorufin, sodium salt *CAS 34994-50-8*
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Additional ordering information
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| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 235.17 |
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
CAS 34994-50-8 | Molecular weight 235.17 | Absorbance (nm) 570 | Extinction coefficient (cm -1 M -1) 650001 | Excitation (nm) 571 | Emission (nm) 584 | Quantum yield 0.751 |
Resorufin is used as a calibration standard for resorufin-derived enzyme substrates. It has pH dependent absorption and emission over the range of 5 to 9. It is also the reduction product of resazurin in cells.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Resorufin, sodium salt *CAS 34994-50-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 425.224 µL | 2.126 mL | 4.252 mL | 21.261 mL | 42.522 mL |
| 5 mM | 85.045 µL | 425.224 µL | 850.449 µL | 4.252 mL | 8.504 mL |
| 10 mM | 42.522 µL | 212.612 µL | 425.224 µL | 2.126 mL | 4.252 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 570 |
| Extinction coefficient (cm -1 M -1) | 650001 |
| Excitation (nm) | 571 |
| Emission (nm) | 584 |
| Quantum yield | 0.751 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0* | 336 | 455 | 5900 | 0.107 |
| Resazurin, sodium salt *CAS 62758-13-8* | 571 | 584 | - | - |
Images
Figure 1. Chemical structure for Resorufin, sodium salt *CAS 34994-50-8*
References
View all 31 references: Citation Explorer
Methoxy-resorufin ether as an electrochemically active biological probe for cytochrome P450 O-demethylation
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Authors: Jenkins AT, Dash HA, Boundy S, Halliwell CM, ffrench-Constant RH.
Journal: Bioelectrochemistry (2006): 67
Induction of ethoxy-resorufin-O-deethylase activity by halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons in primary hepatocytes of the green frog (Rana esculenta)
Authors: Rankouhi TR, Koomen B, S and erson JT, Bosveld AT, Seinen W, van den Berg M.
Journal: Environ Toxicol Chem (2005): 1428
Authors: Rankouhi TR, Koomen B, S and erson JT, Bosveld AT, Seinen W, van den Berg M.
Journal: Environ Toxicol Chem (2005): 1428
Validation and diagnostic efficacy of a lipase assay using the substrate 1,2-o-dilauryl-rac-glycero glutaric acid-(6' methyl resorufin)-ester for the diagnosis of acute pancreatitis in dogs
Authors: Graca R, Messick J, McCullough S, Barger A, Hoffmann W.
Journal: Vet Clin Pathol (2005): 39
Authors: Graca R, Messick J, McCullough S, Barger A, Hoffmann W.
Journal: Vet Clin Pathol (2005): 39
Quantitative structure-activity relationships within a homologous series of 7-alkoxyresorufins exhibiting activity towards CYP1A and CYP2B enzymes: molecular modelling studies on key members of the resorufin series with CYP2C5-derived models of human CYP1A
Authors: Lewis DF, Lake BG, Dickins M.
Journal: Xenobiotica (2004): 501
Authors: Lewis DF, Lake BG, Dickins M.
Journal: Xenobiotica (2004): 501
A resorufin-based fluorescent assay for quantifying NADH
Authors: Batchelor RH, Zhou M.
Journal: Anal Biochem (2002): 118
Authors: Batchelor RH, Zhou M.
Journal: Anal Biochem (2002): 118
Functional visualization of the excretory system of adult Schistosoma mansoni by the fluorescent marker resorufin
Authors: Sato H, Kusel JR, Thornhill J.
Journal: Parasitology (2002): 527
Authors: Sato H, Kusel JR, Thornhill J.
Journal: Parasitology (2002): 527
The excited-state interaction of resazurin and resorufin with amines in aqueous solutions. Photophysics and photochemical reactions
Authors: Bueno C, Villegas ML, Bertolotti SG, Previtali CM, Neumann MG, Encinas MV.
Journal: Photochem Photobiol (2002): 385
Authors: Bueno C, Villegas ML, Bertolotti SG, Previtali CM, Neumann MG, Encinas MV.
Journal: Photochem Photobiol (2002): 385
Hydrogen peroxide-induced deacetylation of acetyl resorufin as a novel indicator reaction for fluorometric detection of glucose using only glucose oxidase
Authors: Maeda H, Matsu-ura S, Nishida M, Senba T, Yamauchi Y, Ohmori H.
Journal: Chem Pharm Bull (Tokyo) (2001): 294
Authors: Maeda H, Matsu-ura S, Nishida M, Senba T, Yamauchi Y, Ohmori H.
Journal: Chem Pharm Bull (Tokyo) (2001): 294
Resorufin as an electron acceptor in glucose oxidase-catalyzed oxidation of glucose
Authors: Maeda H, Matsu-ura S, Senba T, Yamasaki S, Takai H, Yamauchi Y, Ohmori H.
Journal: Chem Pharm Bull (Tokyo) (2000): 897
Authors: Maeda H, Matsu-ura S, Senba T, Yamasaki S, Takai H, Yamauchi Y, Ohmori H.
Journal: Chem Pharm Bull (Tokyo) (2000): 897
Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay
Authors: Henke E, Bornscheuer UT.
Journal: Biol Chem (1999): 1029
Authors: Henke E, Bornscheuer UT.
Journal: Biol Chem (1999): 1029