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Dansyl-X, acid
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 364.46 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.387 |
| Extinction coefficient (cm -1 M -1) | 3300 |
| Excitation (nm) | 335 |
| Emission (nm) | 518 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
Molecular weight 364.46 | Correction Factor (280 nm) 0.387 | Extinction coefficient (cm -1 M -1) 3300 | Excitation (nm) 335 | Emission (nm) 518 |
Compared to Dansyl chloride, Dansyl-X acid gives better yield for labeling peptides and nucleotides. Dansyl dyes have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl dyes an important tool for biophysical studies. They are particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Dansyl-X, acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 274.379 µL | 1.372 mL | 2.744 mL | 13.719 mL | 27.438 mL |
| 5 mM | 54.876 µL | 274.379 µL | 548.757 µL | 2.744 mL | 5.488 mL |
| 10 mM | 27.438 µL | 137.189 µL | 274.379 µL | 1.372 mL | 2.744 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.387 |
| Extinction coefficient (cm -1 M -1) | 3300 |
| Excitation (nm) | 335 |
| Emission (nm) | 518 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Dansyl-X, SE | 335 | 518 | 3300 |
| NBD-X acid *CAS 88235-25-0* | 467 | 539 | 22000 |
Images
Figure 1. Chemical structure for Dansyl-X, acid
References
View all 33 references: Citation Explorer
Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma
Authors: Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1
Authors: Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1
Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine
Authors: Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.
Journal: J Anal Toxicol (2002): 17
Authors: Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.
Journal: J Anal Toxicol (2002): 17
Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride
Authors: Naassner M, Mergler M, Wolf K, Schuphan I.
Journal: J Chromatogr A (2002): 133
Authors: Naassner M, Mergler M, Wolf K, Schuphan I.
Journal: J Chromatogr A (2002): 133
Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2
Authors: Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.
Journal: Analyst (1998): 2871
Authors: Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.
Journal: Analyst (1998): 2871
A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence
Authors: Yamada H, Ikeda-Wada S, Oguri K.
Journal: Biol Pharm Bull (1998): 778
Authors: Yamada H, Ikeda-Wada S, Oguri K.
Journal: Biol Pharm Bull (1998): 778
Determination of L-buthionin (SR)-sulfoximine, gamma-glutamylcysteine synthetase inhibitor in rat plasma with HPLC after prelabeling with dansyl chloride
Authors: Koyama H, Sugioka N, Hirata I, Ohta T, Kishimoto H.
Journal: J Chromatogr Sci (1996): 326
Authors: Koyama H, Sugioka N, Hirata I, Ohta T, Kishimoto H.
Journal: J Chromatogr Sci (1996): 326
Dansyl chloride labelling of stratum corneum: its rapid extraction from skin can predict skin irritation due to surfactants and cleansing products
Authors: Paye M, Simion FA, Pierard GE.
Journal: Contact Dermatitis (1994): 91
Authors: Paye M, Simion FA, Pierard GE.
Journal: Contact Dermatitis (1994): 91
Dihydroxyacetone test as a substitute for the dansyl chloride test
Authors: Pierard GE, Pierard-Franchimont C.
Journal: Dermatology (1993): 133
Authors: Pierard GE, Pierard-Franchimont C.
Journal: Dermatology (1993): 133
Microscopic evaluation of the dansyl chloride test
Authors: Pierard GE., undefined
Journal: Dermatology (1992): 37
Authors: Pierard GE., undefined
Journal: Dermatology (1992): 37
Determination of the insecticide promecarb by fluorogenic labelling with dansyl chloride
Authors: Garcia Sanchez F, Cruces Blanco C.
Journal: Analyst (1991): 851
Authors: Garcia Sanchez F, Cruces Blanco C.
Journal: Analyst (1991): 851