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NBD-X, succinimidyl ester *CAS 145195-58-0*

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Physical properties
Molecular weight391.33
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)22000
Excitation (nm)467
Emission (nm)539
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

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CAS
145195-58-0
Molecular weight
391.33
Extinction coefficient (cm -1 M -1)
22000
Excitation (nm)
467
Emission (nm)
539
Compared to NBD chloride and fluoride, NBD-X acid gives better yield for labeling biopolymers. The fluorescence spectra of NBD derivatives are highly environment-sensitive, the fluorescence intensity decreases significantly in aqueous solutions.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of NBD-X, succinimidyl ester *CAS 145195-58-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM255.539 µL1.278 mL2.555 mL12.777 mL25.554 mL
5 mM51.108 µL255.539 µL511.078 µL2.555 mL5.111 mL
10 mM25.554 µL127.769 µL255.539 µL1.278 mL2.555 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)22000
Excitation (nm)467
Emission (nm)539

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Citations


View all 2 citations: Citation Explorer
Comparison among different “revealers” in the study of accelerated blood clearance phenomenon
Authors: Liang, Kaifan and Wang, Lirong and Su, Yuqing and Liu, Mengyang and Feng, Rui and Song, Yanzhi and Deng, Yihui
Journal: European Journal of Pharmaceutical Sciences (2018): 210--216
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)

References


View all 102 references: Citation Explorer
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Two novel missense and one novel nonsense CFTR mutations in Iranian males with congenital bilateral absence of the vas deferens
Authors: Radpour R, Gourabi H, Gilani MA, Dizaj AV, Rezaee M, Mollamohamadi S.
Journal: Mol Hum Reprod (2006): 717
In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer
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Journal: Embo J (2006): 4728
Simultaneous liquid chromatographic assay of amantadine and its four related compounds in phosphate-buffered saline using 4-fluoro-7-nitro-2,1,3-benzoxadiazole as a fluorescent derivatization reagent
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Journal: Biomed Chromatogr (2006): 423
ABNORMAL REGULATORY INTERACTIONS OF I148T-CFTR AND THE EPITHELIAL Na+ CHANNEL IN XENOPUS OOCYTES
Authors: Suaud L, Yan W, Rubenstein RC.
Journal: Am J Physiol Cell Physiol. (2006)
Tris(pyrazolyl)borate carbosilane dendrimers and metallodendrimers
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Journal: Dalton Trans (2006): 5287
Conformational and functional characterization of trapped complexes of the P-glycoprotein multidrug transporter
Authors: Russell PL, Sharom FJ.
Journal: Biochem J (2006): 315
Domain Interdependence in the Biosynthetic Assembly of CFTR
Authors: Cui L, Aleks and rov L, Chang XB, Hou YX, He L, Hegedus T, Gentzsch M, Aleks and rov A, Balch WE, Riordan JR.
Journal: J Mol Biol. (2006)
Nucleotide binding domains of human CFTR: a structural classification of critical residues and disease-causing mutations
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Journal: Cell Mol Life Sci (2005): 2112