Bromocresol purple *CAS 115-40-2* *Ultrapure grade*
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Additional ordering information
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 540.22 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Room temperature (10-25 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Intracellular pH
CAS 115-40-2 | Molecular weight 540.22 |
Bromocresol purple (BCP) or 5',5-dibromo-o-cresolsulfophthalein, is a pH indicator. In its sultone form, it has a pKa value of 6.3, and is usually prepared as a 0.04% aqueous solution. Besides its primary function as an indicator, bromocresol purple is used in medical laboratories to measure albumin.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Bromocresol purple *CAS 115-40-2* *Ultrapure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 185.11 µL | 925.549 µL | 1.851 mL | 9.255 mL | 18.511 mL |
5 mM | 37.022 µL | 185.11 µL | 370.22 µL | 1.851 mL | 3.702 mL |
10 mM | 18.511 µL | 92.555 µL | 185.11 µL | 925.549 µL | 1.851 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 32 references: Citation Explorer
Albumin concentration determined by the modified bromocresol purple method is superior to that by the bromocresol green method for assessing nutritional status in malnourished patients with inflammation
Authors: Ueno T, Hirayama S, Ito M, Nishioka E, Fukushima Y, Satoh T, Idei M, Horiuchi Y, Shoji H, Ohmura H, Shimizu T, Miida T.
Journal: Ann Clin Biochem (2013): 576
Authors: Ueno T, Hirayama S, Ito M, Nishioka E, Fukushima Y, Satoh T, Idei M, Horiuchi Y, Shoji H, Ohmura H, Shimizu T, Miida T.
Journal: Ann Clin Biochem (2013): 576
Using polyvinyl chloride dyed with bromocresol purple in radiation dosimetry
Authors: Kattan M, al Kassiri H, Daher Y.
Journal: Appl Radiat Isot (2011): 377
Authors: Kattan M, al Kassiri H, Daher Y.
Journal: Appl Radiat Isot (2011): 377
Serum globulins contribute to the discrepancies observed between the bromocresol green and bromocresol purple assays of serum albumin concentration
Authors: Xu Y, Wang L, Wang J, Liang H, Jiang X.
Journal: Br J Biomed Sci (2011): 120
Authors: Xu Y, Wang L, Wang J, Liang H, Jiang X.
Journal: Br J Biomed Sci (2011): 120
Mechanism for the color change in bromocresol purple bound to human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 294
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 294
Identification of two bromocresol purple binding sites on human serum albumin
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 1536
Authors: Ito S, Yamamoto D.
Journal: Clin Chim Acta (2010): 1536
Spectroscopic studies on the binding of bromocresol purple to different serum albumins and its bilirubin displacing action
Authors: Faizul FM, Abdul Kadir H, Tayyab S.
Journal: J Photochem Photobiol B (2008): 1
Authors: Faizul FM, Abdul Kadir H, Tayyab S.
Journal: J Photochem Photobiol B (2008): 1
Extractive spectrophotometric methods for the determination of oxomemazine hydrochloride in bulk and pharmaceutical formulations using bromocresol green, bromocresol purple and bromophenol blue
Authors: El-Didamony AM., undefined
Journal: Arch Pharm (Weinheim) (2005): 190
Authors: El-Didamony AM., undefined
Journal: Arch Pharm (Weinheim) (2005): 190
Spectrophotometric determination of indinavir in bulk and pharmaceutical formulations using bromocresol purple and bromothymol blue
Authors: Erk N., undefined
Journal: Pharmazie (2004): 183
Authors: Erk N., undefined
Journal: Pharmazie (2004): 183
Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods
Authors: Duly EB, Grimason S, Grimason P, Barnes G, Trinick TR.
Journal: J Clin Pathol (2003): 780
Authors: Duly EB, Grimason S, Grimason P, Barnes G, Trinick TR.
Journal: J Clin Pathol (2003): 780
Spectrophotometric determination of enoxacin as ion-pairs with bromophenol blue and bromocresol purple in bulk and pharmaceutical dosage form
Authors: Suslu I, Tamer A.
Journal: J Pharm Biomed Anal (2002): 545
Authors: Suslu I, Tamer A.
Journal: J Pharm Biomed Anal (2002): 545
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
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A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
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I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
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What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL
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RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview