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DBCO-Cy5

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Physical properties
Molecular weight1211.60
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


See also: Cyanines
Molecular weight
1211.60
Correction Factor (260 nm)
0.02
Correction Factor (280 nm)
0.03
Correction Factor (482 nm)
0.009
Correction Factor (565 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
651
Emission (nm)
670
Quantum yield
0.271, 0.42
This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. AAT Bioquest offer a broad range of dye azide compounds (such as coumarin azides, fluorescein azides, rhodamine azides and cyanine azides) for click reactions.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy5 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM82.535 µL412.677 µL825.355 µL4.127 mL8.254 mL
5 mM16.507 µL82.535 µL165.071 µL825.355 µL1.651 mL
10 mM8.254 µL41.268 µL82.535 µL412.677 µL825.355 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cy5 tertrazine [Cy5 tertrazine]65167025000010.271, 0.420.020.030.0090.09
DBCO-Cy355556915000010.1510.070.073--
Cy5 phosphoramidite65167025000010.271, 0.420.020.030.0090.09
Cy5 tyramide65167025000010.271, 0.420.020.030.0090.09
Cy5 tetrazine65167025000010.271, 0.420.020.030.0090.09
Cy5 aldehyde65167025000010.271, 0.420.020.030.0090.09
Cy3B DBCO56057112000010.5810.0480.069--

Images


Citations


View all 2 citations: Citation Explorer
Synthesis, characterization, and biological activity of spherical nucleic acid (SNA) constructs for cancer therapy and imaging
Authors: Calabrese, Colin
Journal: (2017)

References


View all 49 references: Citation Explorer
Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522
Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344
Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467
Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378
'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287
Specific and quantitative labeling of biomolecules using click chemistry
Authors: Horisawa K., undefined
Journal: Front Physiol (2014): 457
A dynamic duo: pairing click chemistry and postpolymerization modification to design complex surfaces
Authors: Arnold RM, Patton DL, Popik VV, Locklin J.
Journal: Acc Chem Res (2014): 2999
Applications of azide-based bioorthogonal click chemistry in glycobiology
Authors: Zhang X, Zhang Y.
Journal: Molecules (2013): 7145
Peptide conjugation via CuAAC 'click' chemistry
Authors: Ahmad Fuaad AA, Azmi F, Skwarczynski M, Toth I.
Journal: Molecules (2013): 13148
In situ click chemistry: from small molecule discovery to synthetic antibodies
Authors: Millward SW, Agnew HD, Lai B, Lee SS, Lim J, Nag A, Pitram S, Rohde R, Heath JR.
Journal: Integr Biol (Camb) (2013): 87