Fluo-3, pentapotassium salt

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21017	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluo-3, pentapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.169 µL	520.844 µL	1.042 mL	5.208 mL	10.417 mL
5 mM	20.834 µL	104.169 µL	208.338 µL	1.042 mL	2.083 mL
10 mM	10.417 µL	52.084 µL	104.169 µL	520.844 µL	1.042 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	959.98 g/mol	=	mL	x	mM	=	mmol

Citations

View all 10 citations: Citation Explorer

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons

Authors:

Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei

Journal:

Int J Clin Exp Med (2017): 6861--6868

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection

Authors:

Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun

Journal:

Journal of cellular and molecular medicine (2015): 581--594

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang

Authors:

Rockwell PL, Storey BT.

Journal:

Mol Reprod Dev (2000): 335

Physical properties

Dissociation constant (K_d, nM)	390
Molecular weight	959.98
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	86,000 ¹
Excitation (nm)	506
Emission (nm)	515
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 3, 2025