Home
Calbryte™ series now available!

The future of calcium detection is bright

Video animation comparing fluo-4 am and Calbryte™ 520 performance.
CLICK TO PLAY
ATP response was measured in CHO-K1 cells with Fluo-4, AM (left) and Calbryte™ 520 AM (right). CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10 µg/ml Calbryte™ 520 AM in HH Buffer with probenecid or 10 µg/ml Fluo-4, AM in HH Buffer with probenecid was added and incubated for 45 minutes at 37 °C. Dye loading solution was then removed and replaced with 200 µL HH Buffer/well. ATP (50 µL/well) was added to achieve the final indicated concentration of 10 µM and then imaged with microscope FITC channel (Keyence). Read more



Catalogs and Brochures Biomolecule Quantification Assays & Probes
Calcium Detection Probes & Assay Kits
Cell Analysis Probes & Assay Kits
Cell Apoptosis and Proliferation
Enzyme Probes and Assay Kits
Labeling Antibodies and Biopolymers
Nucleic Acid Detection Probes & Assay Kits
Oligonucleotide Labeling Reagents
Peptide Labeling Reagents
Physiological Probes and Assay Kits
Reactive Oxygen Species (ROS) Detection
Secondary Detection Probes & Kits
Product Application Notes Biotin labeling
Detecting hydroxyl radicals in live cells
iFluor™-IgG conjugates
Live mitochondria ROS
Multi-color functional analysis with calcein
NAD and its detection
Peptide labeling and detection
Phycobiliproteins and flow cytometry
Probeniceid-free, no wash calcium assays
Protein kinase activity assays
Ratiometric calcium detection
Tide Quencher™ for FRET platforms
Recent Product Citations Amplite™ Acetylcholinesterase Assay
Amplite™ Glucose Assay
Amplite™ Hydrogen Peroxide Assay
Amplite™ LOX Activity Assay
Amplite™ NAD/NADH and NADP/NADPH Assays
Cal-520™ Calcium Indicators
CytoTell™ Cell Proliferation Dyes
Fluo-8® Calcium Indicators
JC-10 Apoptosis Probe
Rhod-4™ Calcium Indicators
Screen Quest™ Fluo-8® Calcium Assays
Amplite™ Universal MMP Assay