Calbryte™ series now available!

The future of calcium detection is bright

ATP response was measured in CHO-K1 cells with Fluo-4, AM (left) and Calbryte™ 520 AM (right). CHO-K1 cells were seeded overnight at 50,000 cells/100 µL/well in a 96-well black wall/clear bottom costar plate. 100 µL of 10 µg/ml Calbryte™ 520 AM in HH Buffer with probenecid or 10 µg/ml Fluo-4, AM in HH Buffer with probenecid was added and incubated for 45 minutes at 37 °C. Dye loading solution was then removed and replaced with 200 µL HH Buffer/well. ATP (50 µL/well) was added to achieve the final indicated concentration of 10 µM and then imaged with microscope FITC channel (Keyence). Read more

Catalogs and Brochures Biomolecule Quantification Assays & Probes
Cell Analysis Probes & Assay Kits
Cell Apoptosis and Proliferation
Enzyme Probes and Assay Kits
Fluorescence Antibody Labeling
Nucleic Acid Detection Probes & Assay Kits
Oligonucleotide Labeling Reagents
Peptide Labeling Reagents
Physiological Probes and Assay Kits
Product Application Notes Buccutite™ Rapid Protein Crosslinking
Cal-520™ Calcium Assay Reagents
CytoTell™ Cell Proliferation Reagents
Fluo-8® Calcium Reagents and Kits
Multiplex Cell Proliferation & Toxicity
Quest Rhod-4™ for Calcium Detection
Rapid Maleimide Quantification
Detection of Hydroxyl Radical in Cells
Recent Product Citations Amplite™ Glucose Assay
Amplite™ LOX Activity Assay
Amplite™ NAD/NADH and NADP/NADPH Assays
Cal-520™ Calcium Indicators
CytoTell™ Cell Proliferation Dyes
Fluo-8® Calcium Indicators
JC-10 Apoptosis Probe
Rhod-4™ Calcium Indicators
Screen Quest™ Fluo-8® Calcium Assays