iFluorAlpha FluorReadiLink Rapid iFluortrFluorReadiLink

iFluor Dyes and Kits

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AAT Bioquest iFluor™ dyes are a series of highly water-soluble fluorescent dyes for labeling biomolecules, such as proteins and antibodies. Available in a variety of excitation-emission profiles, spanning the UV-visible to near-infrared spectrum, there is an iFluor™ dye to match any instrument set-up and many combinations for multiplex detection. Conjugates made with iFluor™ dyes exhibit superior brightness and photostability, outperforming most Alexa Fluor® conjugates and other spectrally similar conjugates. Use iFluor™ products for all your immunofluorescence assays, cell imaging and flow cytometric applications.


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HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by iFluor™ 488 goat anti-mouse IgG conjugate (Green, Left) or Alexa Fluor® 488 goat anti-mouse IgG conjugate (Green, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue).

iFluor™  Secondary Reagents

iFluor™ secondary conjugates are produced with the highest quality antibodies and streptavidins. To ensure our customers receive products with the greatest sensitivity and selectivity, our secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity. The excellent photostability and pH-insensitive fluorescence of our iFluor™ secondary conjugates, allows researchers more time for capturing images.

 

Reactive Dyes

iFluor™ reactive dyes may be covalently labeled to biomolecules without self-quenching, producing intensely fluorescent conjugates. iFluor™ reactive dye formats include amine-reactive succinimidyl ester, thiol-reactive maleimide, and more. For assistance labeling proteins with succinimidyl ester or maleimide iFluor™ dyes, use our online interactive protein labeling protocol.

 

Labeling Kits

ReadiLink™ Rapid iFluor™ labeling kits offer a robust and simplified method for labeling antibodies in microscale. Produce iFluor™ antibody conjugates in two mixing steps without any column purification.

 

Bioconjugates

We offer a large selection of iFluor™-labeled conjugates for a wide range of applications. Featured conjugates include, Annexin V for apoptic detection and phalloidin for cell structure labeling.

 

iFluor™ Dyes - Emission Spectra

 

  
Click For Spectrum:
1. iFluor™ 350
2. iFluor™ 405
3. iFluor™ 430
4. iFluor™ 450
5. iFluor™ 488
6. iFluor™ 514
7. iFluor™ 532
8. iFluor™ 546
9. iFluor™ 555
  
10. iFluor™ 568
11. iFluor™ 594
12. iFluor™ 610
13. iFluor™ 633
14. iFluor™ 647
15. iFluor™ 660
16. iFluor™ 680
17. iFluor™ 700
18. iFluor™ 750
  
19. iFluor™ 790
20. iFluor™ 800
21. iFluor™ 810
22. iFluor™ 820
23. iFluor™ 860

Figure 1. Top) Emission spectra of ultraviolet and blue light-excitable iFluor™ dyes. Middle) Emission spectra of green, yellow and red light-excitable iFluor™ dyes. Bottom) Emission spectra of far-red and infrared light-excitable iFluor™ dyes.


ADDITIONAL RESOURCES

Table 1. Spectral properties of iFluor™ fluorescent labeling dyes.

 Dye  Ext. Coeff. 1  Abs. (nm)  Em. (nm)  FQY 2  CF at 260 nm 3  CF at 280 nm 4
 iFluor™ 350  20,000  346  443  0.95  0.83  0.23
 iFluor™ 405  37,000  402  425  0.91  0.48  0.77
 iFluor™ 430  40,000  433  497  0.78  0.68  0.3
 iFluor™ 450  40,000  449  504  0.82  0.45  0.27
 iFluor™ 488  75,000  491  518  0.9  0.21  0.11
 iFluor™ 514  80,000  511  527  0.83  0.25  0.11
 iFluor™ 532  90,000  536  560  0.68  0.26  0.16
 iFluor™ 546  100,000  541  557  0.67  0.25  0.15
 iFluor™ 555  100,000  552  567  0.64  0.23  0.14
 iFluor™ 568  100,000  568  587  0.57  0.34  0.15
 iFluor™ 594  180,000  594  614  0.53  0.05  0.04
 iFluor™ 610  110,000  605  627  0.85  0.32  0.49
 iFluor™ 633  250,000  638  655  0.29  0.062  0.044
 iFluor™ 647  250,000  649  664  0.25  0.03  0.03
 iFluor™ 660  250,000  662  678  0.26  0.07  0.08
 iFluor™ 680  220,000  684  702  0.23  0.097  0.094
 iFluor™ 700  220,000  685  710  0.23  0.09  0.04
 iFluor™ 710  190,000  712  736  0.14  0.12  0.07
 iFluor™ 750  275,000  749  775  0.12  0.044  0.039
 iFluor™ 790  275,000  782  811  0.13  0.1  0.09
 iFluor™ 800  250,000  801  820  0.11  0.03  0.08
 iFluor™ 810  250,000  811  825  0.05  0.09  0.15
 iFluor™ 820  250,000  822  852  N/A  0.11  0.16
 iFluor™ 860  250,000  854  878  N/A  0.1  0.14

Note:

  1. Ext. Coeff. = Extinction coefficient at their maximum absorption wavelength. The units of extinction coefficient are cm-1M-1.
  2. FQY = fluorescence quantum yield in aqueous buffer (pH 7.2).
  3. CF at 260 nm is the correction factor used for eliminating the dye contribution to the absorbance at 260 nm.
  4. CF at 280 nm is the correction factor used for eliminating the dye contribution to the absorbance at 280 nm (for peptides and protein labeling).
  5. Fluorescence intensity is significantly increased upon coupling to proteins.

 

Table 2. iFluor™ dye equivalents of common dyes.

 If you are using  Try this iFluor™ Dye
 Alexa Fluor® 350, AMCA, coumarin  iFluor™ 350
 Alexa Fluor® 488, Atto 488, HiLyte™ 488, Cy2, FITC  iFluor™ 488
 Alexa Fluor® 555, Atto 550, HiLyte™ 555, Cy3, TRITC  iFluor™ 555
 Alexa Fluor® 568, Atto 565, Rhodamine Red  iFluor™ 568
 Alexa Fluor® 594, Atto 594, Texas Red  iFluor™ 594
 Alexa Fluor® 647, Atto 647, HiLyte™ 647, Cy5  iFluor™ 647
 Alexa Fluor® 680, Atto 680 Cy5.5, IRDye® 680  iFluor™ 680
 Alexa Fluor® 750, HiLyte™ 750, Cy7  iFluor™ 750

Note:

  1. Alexa Fluor®  is a registered trademark of ThermoFisher.
  2. HiLyte™ is a trademark of AnaSpec.

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