Name: iFluor® Ultra 750 succinimidyl ester
Brand: AAT Bioquest
SKU: 71680
Availability: InStock

iFluor® Ultra 750 succinimidyl ester

Fluorescent dye-conjugated antibodies provide a tool for identifying proteins in many applications including fluorescent cell imaging, flow cytometry, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled antibody include higher sensitivity, multiplexing capabilities, and ease of use. iFluor® Ultra family is a recent upgrade of our popular iFluor® dyes and optimized for labeling antibodies used for fluorescence imaging and flow cytometry applications. Antibody conjugates prepared with iFluor® Ultra 750 are far superior to the conjugates of other existing similar dyes such as Cy7, Dylight 755, IRDye750 and Alexa Fluor® 750. iFluor® Ultra 750 conjugates might be the brightest among the NIR dye antibody conjugates. They are significantly brighter than the conjugates prepared with Cy7, Dylight 755, IRDye750 and Alexa Fluor® 750 under the same conditions. Additionally, the fluorescence of iFluor® Ultra 750 is not affected by pH (4-10). iFluor® Ultra 750 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® Ultra 750 has spectral properties and reactivity similar to Cy7, Dylight 755, IRDye750 and Alexa Fluor® 750.

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> Ultra 750 goat anti-mouse IgG conjugate or Alexa Fluor<sup>®</sup> 750 goat anti-mouse IgG.

Protocol

COA

Catalog	Size	Price
71680	1 mg	Price
71681	100 ug	Price
71682	5 mg	Price

References

View all 28 references: Citation Explorer

Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging.

Authors:

Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C

Journal:

Analytical chemistry (2020)

CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.

Authors:

Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and de Jalón, Elvira García and Tenstad, Olav and Bjørge, Line and McCormack, Emmet

Journal:

EBioMedicine (2020): 102783

Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging.

Authors:

Duranti, Claudia and Carraresi, Laura and Sette, Angelica and Stefanini, Matteo and Lottini, Tiziano and Crescioli, Silvia and Crociani, Olivia and Iamele, Luisa and De Jonge, Hugo and Gherardi, Ermanno and Arcangeli, Annarosa

Journal:

Oncotarget (2018): 34972-34989

Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol).

Authors:

Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania

Journal:

ACS applied materials & interfaces (2018): 30071-30080

Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging.

Authors:

Dong, Lili and Zhou, Hongxin and Zhao, Menglong and Gao, Xinghui and Liu, Yang and Liu, Dongli and Guo, Wei and Hu, Hongwei and Xie, Qian and Fan, Jia and Lin, Jiang and Wu, Weizhong

Journal:

Molecular therapy. Nucleic acids (2018): 376-386

Physical properties

Molecular weight	1426.78
Solvent	DMSO

Spectral properties

Absorbance (nm)	750
Correction factor (260 nm)	0.04
Correction factor (280 nm)	0.05
Correction factor (565 nm)	0.021
Correction factor (650 nm)	0.149
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	749
Emission (nm)	773
Quantum yield	0.32 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025