AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. iFluor® 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor® 594 family has the spectral properties similar to those of Texas Red® and Alexa Fluor® 594 (Texas Red® and Alexa Fluor® 594 are the trademarks of Invitrogen). iFluor® 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative to Texas Red® and Alexa Fluor® 594.Compared to Texas Red®, iFluor® 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor® 594 tandem has better FRET efficiency.ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 594 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 594 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 594 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 594 SE.