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ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's iFluor® dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor® 700 dyes have fluorescence excitation and emission maxima close to 690 nm and 710 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 700 (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 700 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 700 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 700 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 700 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

HL-60 cells were incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit (Cat No. 1245). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
HL-60 cells were incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit (Cat No. 1245). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
CatalogSize
Price
Quantity
12452 Labelings
Price
 
Spectral properties

Correction factor (260 nm)0.09
Correction factor (280 nm)0.04
Correction factor (565 nm)0.0766
Correction factor (650 nm)0.4004
Extinction coefficient (cm -1 M -1)
220000
1
Excitation (nm)690
Emission (nm)713
Quantum yield
0.23
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 21, 2025