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ReadiLink™ BSA Conjugation Kit
Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Like most abundant plasma proteins, it is very stable and soluble. In addition, the 67 kDa protein is sufficiently large and complex to be fully immunogenic. It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. Consequently, BSA is a popular carrier protein for conjugation to haptens and other weak antigens to make them more immunogenic for antibody production. This ReadiLink™ BSA Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. The ReadiLink™ BSA Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. These kits contain BSA formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step.
<p>EDC reacts with a carboxyl group of carrier protein BSA or KLH (represented by the red ball), forming an amine-reactive O-acylisourea intermediate (the central molecule). The O-acylisourea intermediate reacts with an amine group on the antigen molecule represented by the smaller blue ball, yielding a conjugate of the two molecules joined by a stable amide bond [Please note the O-acylisourea inermediate is also susceptible to hydrolysis, making it unstable and short-lived in aqueous solution].</p>
<p>EDC reacts with a carboxyl group of carrier protein BSA or KLH (represented by the red ball), forming an amine-reactive O-acylisourea intermediate (the central molecule). The O-acylisourea intermediate reacts with an amine group on the antigen molecule represented by the smaller blue ball, yielding a conjugate of the two molecules joined by a stable amide bond [Please note the O-acylisourea inermediate is also susceptible to hydrolysis, making it unstable and short-lived in aqueous solution].</p>
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
55012 Reactions
Price
 
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
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Page updated on October 13, 2025