logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 680 dyes have fluorescence excitation and emission maxima close to 680 nm and 700 nm respectively with good photostability. These spectral characteristics make them an excellent alternative to Cy5.5®, IRDye® 700 and Alexa Fluor® 680 (Cy5.5®, IRDye® and Alexa Fluor® are the trademarks of GE Healthcare, Li-COR and Invitrogen respectively). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 680 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 680 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 680 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 680 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Flow cytometric analysis of cells undergoing apoptosis using Annexin V-iFluor® 680. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37 ºC. Cells were then incubated with Annexin V labeled using the ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit (Cat No. 1240) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.
Flow cytometric analysis of cells undergoing apoptosis using Annexin V-iFluor® 680. Jurkat cells were treated with (red) or without 1 µM staurosporine (green) for 4 hours at 37 ºC. Cells were then incubated with Annexin V labeled using the ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit (Cat No. 1240) for 30 minutes to identify apoptotic cells. Fluorescence intensity was measured using an ACEA NovoCyte flow cytometer.
CatalogSize
Price
Quantity
12402 Labelings
Price
 
Spectral properties

Correction factor (260 nm)0.097
Correction factor (280 nm)0.094
Extinction coefficient (cm -1 M -1)
220000
1
Excitation (nm)684
Emission (nm)701
Quantum yield
0.23
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Contact us

Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on September 24, 2025