Name: ReadiLink™ Protein Biotinylation Kit
Brand: AAT Bioquest
SKU: 5520
Price: 232 USD
Availability: InStock

ReadiLink™ Protein Biotinylation Kit

Biotin is widely used for labeling biomolecules, in particular, antibodies. Our ReadiLink™ Protein Biotinylation Kit is optimized for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). It uses biotin succinimidyl ester that reacts with the amino groups of IgG and other biomolecules. This kit comes with all the necessary reagents for labeling and purification. On our hands, 3 to 6 biotin molecules can be conjugated to each IgG molecule depending on the molecular weight and lysine residues available on the IgG molecule. The kit can be used for 2 conjugation reactions. For each conjugation reaction the material can label up to 1 mg protein. The entire process only takes less than an hour.

Catalog	Size	Price	Quantity
5520	2 Labelings	Price

Citations

View all 4 citations: Citation Explorer

Costunolide covalently targets NACHT domain of NLRP3 to inhibit inflammasome activation and alleviate NLRP3-driven inflammatory diseases

Authors:

Xu, Haowen and Chen, Jiahao and Chen, Pan and Li, Weifeng and Shao, Jingjing and Hong, Shanshan and Wang, Yi and Chen, Lingfeng and Luo, Wu and Liang, Guang

Journal:

Acta Pharmaceutica Sinica B (2022)

A low-cost, high-performance system for fluorescence lateral flow assays

Authors:

Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F

Journal:

Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms

Authors:

Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor

Journal:

Biomacromolecules (2010): 2804--2809

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields

Authors:

Morotomi-Yano, Keiko and Yano, Ken-ichi

Journal:

FEBS Open Bio

References

View all 78 references: Citation Explorer

Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))

Authors:

Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.

Journal:

Eur J Nucl Med Mol Imaging. (2006)

A biotin-protein bond with stability in plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2005): 98

Probing the interaction of the biotin-avidin complex with the relaxivity of biotinylated Gd-DTPA

Authors:

Langereis S, Kooistra HA, van Genderen MH, Meijer EW.

Journal:

Org Biomol Chem (2004): 1271

Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology

Authors:

Huhtinen P, Soukka T, Lovgren T, Harma H.

Journal:

J Immunol Methods (2004): 111

Biotin is endogenously expressed in select regions of the rat central nervous system

Authors:

McKay BE, Molineux ML, Turner RW.

Journal:

J Comp Neurol (2004): 86

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

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Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025

Component A: ReadiLink™ Biotin SE	2 vials (lyophilized)
Component B: Reaction Buffer	1 vial (200 µL)
Component C: Spin Column	2 columns