AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 350 dyes have fluorescence excitation and emission maxima of ~350 nm and ~440 nm respectively. These spectral characteristics make them an excellent replacement for AMCA and Alexa Fluor® 350 labeling dye (Alexa Fluor® is the trademark of Invitrogen). ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 350 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 350 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 350 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 350 SE.