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ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's iFluor® dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. iFluor® 790 dyes have fluorescence excitation and emission maxima close to 790 nm and 810 nm respectively. These spectral characteristics make them an excellent alternative to IRDye® 800 (IRDye® is the trademark of Li-COR). Our in-house comparable studies indicated that our iFluor 790 dyes are significantly brighter than the corresponding IRDye® 800. iFluor® 790 conjugates have been widely used in fluorescence animal imaging applications. ReadiLink™ labeling kits essentially only require 2 simple mixing steps without a column purification needed. iFluor® 790 SE used in this ReadiLink™ kit is reasonably stable and shows good reactivity and selectivity with protein amino groups. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of iFluor® 790 dye provided in the kit is optimized for labeling ~50 µg antibody. iFluor® 790 SE protein labeling kit provides a convenient method to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the iFluor® 790 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

HL-60 cells were incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit (Cat No. 1265). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC-Cy7 channel.
HL-60 cells were incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit (Cat No. 1265). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC-Cy7 channel.
CatalogSize
Price
Quantity
12652 Labelings
Price
 
Spectral properties

Correction factor (260 nm)0.1
Correction factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)787
Emission (nm)812
Quantum yield
0.13
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on September 24, 2025