Name: ReadiLink™ KLH Conjugation Kit
Brand: AAT Bioquest
SKU: 5502
Availability: InStock

ReadiLink™ KLH Conjugation Kit

ReadiLink™ KLH Conjugation Kit is a one-step conjugation kit for preparing hapten- or peptide-KLH carrier conjugates for immunization and antibody production.

Hapten-carrier conjugation: Enables simple preparation of hapten- or peptide-carrier conjugates using mcKLH as the carrier protein
Carbodiimide coupling: Uses carboxyl-reactive carbodiimide chemistry to promote conjugation between carboxyl-containing molecules and available amino groups
Immunogenic carrier protein: Provides mcKLH, a highly immunogenic hemocyanin carrier protein with multiple sites for antigen conjugation
Streamlined cleanup: Includes desalting spin columns for convenient removal of small-molecule reaction components by centrifugation
Research applications: Suitable for preparing immunogens for antibody production against haptens, peptides, and other small antigens

<p>EDC reacts with a carboxyl group of carrier protein BSA or KLH (represented by the red ball), forming an amine-reactive O-acylisourea intermediate (the central molecule). The O-acylisourea intermediate reacts with an amine group on the antigen molecule represented by the smaller blue ball, yielding a conjugate of the two molecules joined by a stable amide bond [Please note the O-acylisourea inermediate is also susceptible to hydrolysis, making it unstable and short-lived in aqueous solution].</p>

Protocol

Catalog	Size	Price	Quantity
5502	2 Reactions	Price
5502-10MG	2 Reactions (Bulk)	Price

Overview

ReadiLink™ KLH Conjugation Kit is designed for the preparation of hapten-carrier and peptide-carrier conjugates for immunization and antibody production. Keyhole limpet hemocyanin (KLH) and related hemocyanin carrier proteins are commonly used in antibody production because their large size and strong immunogenicity help enhance immune responses against small antigens such as haptens and peptides.

The kit uses mcKLH, a hemocyanin carrier protein formulated in buffers compatible with carbodiimide-mediated conjugation chemistry. The carboxyl-reactive carbodiimide activates carboxyl groups on peptides, haptens, or proteins, allowing coupling to available amino groups to form stable amide bonds. ReadiLink™ KLH Conjugation Kit also includes desalting spin columns for convenient cleanup and recovery of the carrier conjugate. The resulting hapten- or peptide-carrier conjugates are suitable for immunization workflows and antibody production against small antigens that are poorly immunogenic on their own.

Components

Catalog 5502 (2 Reactions)

Component A: mcKLH	2 X 2 mg
Component B: Conjugation Buffer (pH 4.7)	1 bottle (20 mL)
Component C: EDC (1-ethy 1-3-[dimethylaminopropyl] carbodiimide hydrochloride)	2 X 10 mg
Component D: Purification Buffer Salts (pH 7.2)	2 X 10 mL
Component E: Spin Desalting Columns (7K MWCO)	2 X 2 mL

Catalog 5502-10MG (2 Reactions (Bulk))

Component A: mcKLH	2 x 10mg
Component B: Conjugation Buffer (pH 4.7)	One bottle (20 mL)
Component C: EDC (1-ethy 1-3-[dimethylaminopropyl] carbodiimide hydrochloride)	2 x 10 mg
Component D: Purification Buffer Salts (pH 7.2)	One bottle (50 mL)
Component E: Spin Desalting Columns (7K MWCO)	2 x 10 mL

Citations

View all 5 citations: Citation Explorer

DEVELOPMENT OF A POINT OF CARE (POC) TEST AS AN IMMUNOBIOSENSOR FOR OKADAIC ACID DETECTION IN MUSSELS

Authors:

Daniso, E and Melpignano, P and Cocchi, M and Susmel, S and Tulli, F

Journal:

Applied Food Research (2025): 100830

Sequential Immunizations with Influenza Neuraminidase Protein Followed by Peptide Nanoclusters Induce Heterologous Protection

Authors:

Song, Wen-Wen and Wan, Mu-Yang and She, Jia-Yue and Zhao, Shi-Long and Liu, De-Jian and Chang, Hai-Yan and Deng, Lei

Journal:

Viruses (2024): 77

Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections

Authors:

Liu, De-Jian and Liu, Cui-Cui and Zhong, Xiu-Qin and Wu, Xuan and Zhang, Hui-Hui and Lu, Shang-Wen and Shen, Zhuo-Ling and Song, Wen-Wen and Zhao, Shi-Long and Peng, You-Song and others,

Journal:

Cell Reports (2023)

Mechanistic Analysis of Influenza A Virus Assembly and Host Gene Regulation

Authors:

Chaimayo, Chutikarn

Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein

Authors:

Chaimayo, Chutikarn and Hayashi, Tsuyoshi and Underwood, Andrew and Hodges, Erin and Takimoto, Toru

Journal:

Virology (2017): 23--32

References

View all 29 references: Citation Explorer

Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)

Authors:

Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.

Journal:

Int J Nanomedicine (2009): 321

Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate

Authors:

Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.

Journal:

J Food Sci (2008): C84

Characterization of bovine serum albumin glycated with glucose, galactose and lactose

Authors:

Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.

Journal:

Acta Biochim Pol (2008): 491

Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio

Authors:

Zheng C, Bi J, Ma G, Su Z.

Journal:

Artif Cells Blood Substit Immobil Biotechnol (2007): 568

Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions

Authors:

Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.

Journal:

Anal Bioanal Chem (2007): 969

Usage and storage

Intended use

Research Use Only (RUO)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 29, 2026