Name: ReadiLink™ KLH Conjugation Kit
Brand: AAT Bioquest
SKU: 5502
Availability: InStock

ReadiLink™ KLH Conjugation Kit

Keyhole Limpet Hemocyanin (KLH) is one of the most commonly used carriers in the conjugation of peptides for antibody production. Mariculture keyhole limpet hemocyanin (mcKLH) is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a carrier protein for the production of antibodies to haptens and peptides. It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers. mcKLH is currently the industry standard for antibody production against a hapten or peptide. This ReadiLink™ KLH Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production. The ReadiLink™ KLH Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten. The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds. These kits contain mcKLH formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns, which offer exceptional protein recovery by simple centrifugation step.

<p>EDC reacts with a carboxyl group of carrier protein BSA or KLH (represented by the red ball), forming an amine-reactive O-acylisourea intermediate (the central molecule). The O-acylisourea intermediate reacts with an amine group on the antigen molecule represented by the smaller blue ball, yielding a conjugate of the two molecules joined by a stable amide bond [Please note the O-acylisourea inermediate is also susceptible to hydrolysis, making it unstable and short-lived in aqueous solution].</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5502	2 Reactions	Price

Citations

View all 5 citations: Citation Explorer

DEVELOPMENT OF A POINT OF CARE (POC) TEST AS AN IMMUNOBIOSENSOR FOR OKADAIC ACID DETECTION IN MUSSELS

Authors:

Daniso, E and Melpignano, P and Cocchi, M and Susmel, S and Tulli, F

Journal:

Applied Food Research (2025): 100830

Sequential Immunizations with Influenza Neuraminidase Protein Followed by Peptide Nanoclusters Induce Heterologous Protection

Authors:

Song, Wen-Wen and Wan, Mu-Yang and She, Jia-Yue and Zhao, Shi-Long and Liu, De-Jian and Chang, Hai-Yan and Deng, Lei

Journal:

Viruses (2024): 77

Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections

Authors:

Liu, De-Jian and Liu, Cui-Cui and Zhong, Xiu-Qin and Wu, Xuan and Zhang, Hui-Hui and Lu, Shang-Wen and Shen, Zhuo-Ling and Song, Wen-Wen and Zhao, Shi-Long and Peng, You-Song and others,

Journal:

Cell Reports (2023)

Mechanistic Analysis of Influenza A Virus Assembly and Host Gene Regulation

Authors:

Chaimayo, Chutikarn

Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein

Authors:

Chaimayo, Chutikarn and Hayashi, Tsuyoshi and Underwood, Andrew and Hodges, Erin and Takimoto, Toru

Journal:

Virology (2017): 23--32

References

View all 29 references: Citation Explorer

Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)

Authors:

Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.

Journal:

Int J Nanomedicine (2009): 321

Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate

Authors:

Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.

Journal:

J Food Sci (2008): C84

Characterization of bovine serum albumin glycated with glucose, galactose and lactose

Authors:

Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.

Journal:

Acta Biochim Pol (2008): 491

Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio

Authors:

Zheng C, Bi J, Ma G, Su Z.

Journal:

Artif Cells Blood Substit Immobil Biotechnol (2007): 568

Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions

Authors:

Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.

Journal:

Anal Bioanal Chem (2007): 969

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

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Page updated on September 25, 2025

Component A: mcKLH	2 X 2 mg
Component B: Conjugation Buffer (pH 4.7)	1 bottle (20 mL)
Component C: EDC (1-ethy 1-3-[dimethylaminopropyl] carbodiimide hydrochloride)	2 X 10 mg
Component D: Purification Buffer Salts (pH 7.2)	2 X 10 mL
Component E: Spin Desalting Columns (7K MWCO)	2 X 2 mL