Name: ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
Brand: AAT Bioquest
SKU: 5521
Price: 232 USD
Availability: InStock

ReadiLink™ Protein Biotinylation Kit

Biotin is widely used for labeling biomolecules, in particular, antibodies. This kit is primarily used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). Our kit uses our ReadiView™ biotin succinimidyl ester (#3059) that reacts with an amino group of IgG and other biomolecules. Our unique biotin contained in the kit carries a color tag for indicating the degree of biotinylation, thus eliminating the troublesome HABA biotinylation determination. This kit contains all of the necessary reagents for labeling and purification. On our hands, 5 to 8 biotin molecules can be conjugated to each IgG molecule using our kit.

<p>ReadiView™ biotin conjugated with specially designed color tag (CT) for easy determination of biotinylation degree. </p>

Catalog	Size	Price	Quantity
5521	3 Reactions	Price

References

View all 62 references: Citation Explorer

Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein

Authors:

Volkmann G, Liu XQ.

Journal:

PLoS One (2009): e8381

Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation

Authors:

Wang J, Cantor AB, Orkin SH.

Journal:

Curr Protoc Stem Cell Biol (2009): Unit1B 5

A multifunctional single-attachment-point reagent for controlled protein biotinylation

Authors:

Garanger E, Weissleder R, Josephson L.

Journal:

Bioconjug Chem (2009): 170

Measuring plasma membrane protein endocytic rates by reversible biotinylation

Authors:

Gabriel L, Stevens Z, Melikian H.

Journal:

J Vis Exp. (2009)

Use of in vivo biotinylation to study protein-protein and protein-DNA interactions in mouse embryonic stem cells

Authors:

Kim J, Cantor AB, Orkin SH, Wang J.

Journal:

Nat Protoc (2009): 506

Spectral properties

Correction factor (280 nm)	0.05
Extinction coefficient (cm ^-1 M ^-1)	15000 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

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Page updated on September 25, 2025

Component A: ReadiView™ Biotin SE	1 vial
Component B: Reaction Buffer	1 vial (200 µL)
Component C: DMSO	1 vial (100 µL)
Component D: Spin Column	3 columns
Component E: Washing Tube (2 mL)	3 tubes
Component F: Collecting Tube (1.5 mL)	3 tubes