iFluor® 546 Styramide

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 546 Styramide is a superior replacement for Alexa Fluor 546 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Microtubules of fixed HeLa cells were labeled with anti-α tubulin mouse mAb followed by HRP-labeled goat anti-mouse IgG (Cat No. 16728). The fluorescence signal was developed using Alexa Fluor® 546 tyramide or iFluor® 546 styramide™ (Cat No. 45025) and detected with a TRITC/Cy3 filter set. iFluor® 546 styramide™ shows significantly higher fluorescence intensity than Alexa Fluor® 546 tyramide under the same conditions.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
45025	100 Slides	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 546 Styramide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	75.376 µL	376.878 µL	753.756 µL	3.769 mL	7.538 mL
5 mM	15.075 µL	75.376 µL	150.751 µL	753.756 µL	1.508 mL
10 mM	7.538 µL	37.688 µL	75.376 µL	376.878 µL	753.756 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1326.69 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis

Authors:

Solheim, Marie H and Stroganov, Sima and Chen, Weiyi and Subagia, P Sicilia and Bauder, Corinna A and Wnuk-Lipinski, Daria and Del R{\'\i}o-Mart{\'\i}n, Almudena and Sotelo-Hitschfeld, Tamara and Beddows, Cait A and Klemm, Paul and others,

Journal:

Cell (2025)

A Cross-Species Atlas of the Dorsal Vagal Complex Reveals Neural Mediators of Cagrilintide′ s Effects on Energy Balance

Authors:

Ludwig, Mette Q and Coester, Bernd and Gordian, Desiree and Hassan, Shad and Tomlinson, Abigail J and Toure, Mouhamadoul Habib and Christensen, Oliver P and Moltke-Prehn, Anja and Brown, Jenny M and Rausch, Dylan M and others,

Journal:

bioRxiv (2025): 2025--01

Membrane progesterone receptor $\gamma$ (paqr5b) is essential for the formation of neurons in the zebrafish olfactory rosette

Authors:

Mustary, Umme Habiba and Maeno, Akiteru and Rahaman, Md Mostafizur and Ali, Md Hasan and Tokumoto, Toshinobu

Journal:

Scientific Reports (2024): 24354

Physical properties

Molecular weight	1326.69
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 12, 2025