Name: iFluor® 445 succinimidyl ester
Brand: AAT Bioquest
SKU: 1027
Price: 237 USD
Availability: InStock

iFluor® 445 succinimidyl ester

iFluor® 445 Succinimidyl Ester is an amine-reactive fluorescent dye with a large Stokes shift for covalent labeling of antibodies and other proteins in fluorescence imaging and flow cytometry applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 447 nm and emission at 559 nm.
Distinct Fluorescence Color: Offers an additional detection color between Alexa Fluor® 430 and 488.
Applications: Suitable for labeling monoclonal and polyclonal antibodies and other proteins larger than 10 kDa.

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature.
(Bottom) Flow cytometry analysis of whole blood stained with iFluor® 445 Anti-human CD4 Antibody (Clone: SK3). The fluorescence signal was monitored using a Cytek Aurora spectral flow cytometer in the iFluor® 445-specific V8-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
1027	1 mg	Price

Overview

iFluor® 445 dyes have fluorescence excitation and emission maxima of ~445 nm and ~560 nm, respectively. The spectral characteristic of large Stokes Shift makes them an excellent choice for fluorescence imaging applications with high sensitivity. iFluor® 445 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. iFluor® 445 SE provides a unique fluorescence color to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for fluorescence imaging and flow cytometric applications. iFluor® 445 dye conjugates are bright, providing an additional color located between Alexa Fluor® 430 and 488 (ThermoFisher). AAT Bioquest's iFluor® dye family provides the largest collection of fluorescent labeling dyes with different spectral properties. They are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, 633, and 647 nm).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 445 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	103.305 µL	516.524 µL	1.033 mL	5.165 mL	10.33 mL
5 mM	20.661 µL	103.305 µL	206.609 µL	1.033 mL	2.066 mL
10 mM	10.33 µL	51.652 µL	103.305 µL	516.524 µL	1.033 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	968.01 g/mol	=	mL	x	mM	=	mmol

References

View all 40 references: Citation Explorer

Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.

Authors:

Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang

Journal:

Bio-protocol (2022): e4441

Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.

Authors:

Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin

Journal:

Biology of reproduction (2022)

Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.

Authors:

Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu

Journal:

Neural regeneration research (2022): 2316-2320

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.

Authors:

Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben

Journal:

Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

Authors:

Ahishali, Bulent and Kaya, Mehmet

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Physical properties

Molecular weight	968.01
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	1.423
Correction factor (280 nm)	0.639
Extinction coefficient (cm ^-1 M ^-1)	30000
Excitation (nm)	447
Emission (nm)	559
Quantum yield	0.176

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 26, 2026