iFluor™ 750 succinimidyl ester

Save 25% on your purchase of iFluor™ 750 succinimidyl ester when you use the promotion code ifluor25. Act quickly, offer expires 05-31-19.Details

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<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor™ 750 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>
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Unit Size: Cat No: Price (USD): Qty:
1 mg 1037 $195


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)749/775
MW1416.83
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Superior Labeling Dyes
iFluor Dyes and Kits
Related Amine-Reactive Probes
Labeling via Amino Groups
AAT Bioquest's iFluor™ dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor™ 750 dyes have fluorescence excitation and emission maxima close to 750 nm and 780 nm respectively with good photostability. Our in-house comparable studies indicated that our iFluor™ 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen respectively). iFluor™ 750 conjugates have been widely used in fluorescence animal imaging applications. The iFluor™ 750 SE dye is reasonably stable and shows good reactivity and selectivity with proteins.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor™ 750 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

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References & Citations

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: D Gao, N Liu, Y Li, Y Zhang, G Liu
Journal: Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Kenneth Wu, Robert A Chong, Qing Yu, Jin Bai, Donald E Spratt, Kevin Ching, Chan Lee, Haibin Miao, Inger Tappin, Jerard Hurwitz
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Helene Piccard, Nele Berghmans, Eva Korpos, Chris Dillen, Ilse Van Aelst, Sandra Li, Erik Martens, Sandra Liekens, Sam Noppen, Jo Van Damme
Journal: International Journal of Cancer (2012): E425--E436






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Fluorescent Labeling Probes & Kits

Application Notes
1. AssayWise Letters 2012, Vol 1(1)

Certificate of Analysis