Name: iFluor® 750 succinimidyl ester
Brand: AAT Bioquest
SKU: 1037
Availability: InStock

iFluor® 750 succinimidyl ester

iFluor® 750 succinimidyl ester is an amine-reactive, near-infrared fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence detection and imaging applications.

Amine-Reactive Labeling: Exhibits good reactivity and selectivity toward protein amino groups.
Spectral Properties: Excitation/emission maxima of 757/779 nm.
Photostability: Provides good photostability and greater brightness than comparable Cy7® and Alexa Fluor® 750 dyes.
Applications: Suitable for preparing fluorescent conjugates for near-infrared animal imaging.

<p>HL-60 cells were incubated with (Red, +) or without (Green, -) Anti-human HLA-ABC (W6/32 mAb), followed by iFluor® 750 goat anti-mouse IgG conjugate. The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in APC-Cy7 channel.</p>

Protocol

Catalog	Size	Price
1037	1 mg	Price
71037	100 ug	Price
71515	5 mg	Price
71565	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are developed for labeling proteins, particularly antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 750 dyes have fluorescence excitation and emission maxima of ~757 nm and ~779 nm, respectively, with good photostability. Our in-house comparable studies indicated that our iFluor® 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen). iFluor® 750 conjugates have been widely used in fluorescence animal imaging applications. The iFluor® 750 SE dye is reasonably stable and shows good reactivity and selectivity with proteins.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 750 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.58 µL	352.9 µL	705.801 µL	3.529 mL	7.058 mL
5 mM	14.116 µL	70.58 µL	141.16 µL	705.801 µL	1.412 mL
10 mM	7.058 µL	35.29 µL	70.58 µL	352.9 µL	705.801 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1416.83 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

In Vivo Visualization of Collagen Transdermal Absorption by a Combined Second-harmonic Generation and Two-photon Excited Fluorescence Method

Authors:

Ma, Shuhua and He, Gaiying and Yang, Weifeng and Wang, Yi and others

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra and Martens, Erik and Liekens, S and ra and Noppen, Sam and Damme, Jo Van and others

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1416.83
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.044
Correction factor (280 nm)	0.039
Correction factor (565 nm)	0.0250
Correction factor (650 nm)	0.1413
Extinction coefficient (cm ^-1 M ^-1)	275000 ¹
Excitation (nm)	757
Emission (nm)	779
Quantum yield	0.12 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 9, 2026