Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 647 Conjugate

Phalloidin-iFluor® 647 Conjugate is a far-red fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of 640/654 nm.
Instrument Compatibility: Compatible with 640 nm laser excitation and Cy5 fluorescence microscope filter sets.
Applications: Suitable for actin cytoskeleton staining and multicolor fluorescence microscopy.

<p>Fluorescence images of HeLa cells stained with Phalloidin-iFluor® 647 Conjugate using fluorescence microscope with a Cy5 filter set (Red). Live cells were first stained with mitochondria dye MitoLite™ Green. After fixation in 4% formaldehyde, cells were labeled with Phalloidin-iFluor® 647 and counterstained with Nuclear Blue™ DCS1 (Cat#17548, Blue).</p>

Protocol

SDS

Catalog	Size	Price	Quantity
23127	300 Tests	Price

Overview

This deep red fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 647-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Phalloidin-iFluor® 647 Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.99 µL	354.95 µL	709.9 µL	3.549 mL	7.099 mL
5 mM	14.198 µL	70.99 µL	141.98 µL	709.9 µL	1.42 mL
10 mM	7.099 µL	35.495 µL	70.99 µL	354.95 µL	709.9 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1408.65 g/mol	=	mL	x	mM	=	mmol

Citations

View all 164 citations: Citation Explorer

Extrusion bioprinting of FibMA--fibrin semi-IPN hydrogel filaments for enhanced skeletal muscle cell alignment

Authors:

Simaan-Yameen, Haneen and Call, Tobias and Kopty, Katia and Notea-Debutton, Noa and Gargioli, Cesare and Seliktar, Dror

Journal:

Biofabrication (2026): 035050

The Antihistamine Astemizole Potentiates the Antitumor Efficacy of Sorafenib in Hepatocellular Carcinoma by Suppressing Proliferation, Metastasis, and Angiogenesis

Authors:

Zhang, Yixuan and Chen, Xin and Yang, Xuting and Wang, Peiyu and Zhang, Xiaoliang and Kong, Dexin and Wang, Ran

Journal:

Current Issues in Molecular Biology (2026): 451

Capsular polysaccharides of Acinetobacter baumannii modulate antimicrobial resistance and innate immune response

Authors:

Klimkaite, Laurita and Kukanauskaite, Giedre and Naujalis, Joris and Ivanauskas, Danas and Grigorjevas, Dominykas and Petrenaite, Greta and Skinkyte, Meda and Porchun, Jekaterina and Zalyte, Egle and Buchovec, Irina and others

Journal:

Scientific Reports (2026)

Dynamic Modulation of the Microenvironment Promotes Functional Maturation of Engineered Tissues

Authors:

Silberman, Eric and Oved, Hadas and Gil, Itay and Marzook, Amgad and Baruch, Ester Sapir and Shechter, Yahel and Shapira, Assaf and Dvir, Tal

Journal:

Advanced Healthcare Materials (2026): e04573

AI driven 3D subcellular RPE map discovers cell state transitions in establishment of apical-basal polarity

Authors:

Ortolan, Davide and Sathe, Pushkar and Volkov, Andrei and Reichert, Dominik and Sebastian, Sheldon and Maminishkis, Arvydas and Schaub, Nicholas J and Ljungquist, Bengt and Bose, Devika and Ferrari, Jorge and others

Journal:

npj Artificial Intelligence (2026): 20

References

View all 127 references: Citation Explorer

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B.

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Processing of the phalloidin proprotein by prolyl oligopeptidase from the mushroom Conocybe albipes

Authors:

Luo H, Hallen-Adams HE, Walton JD.

Journal:

J Biol Chem (2009): 18070

Physical properties

Molecular weight	1408.65
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026