Phalloidin Conjugates | AAT Bioquest

Phalloidin-iFluor® 405 Conjugate

Phalloidin-iFluor® 405 Conjugate is a violet-fluorescent probe designed for selective detection and visualization of filamentous actin (F-actin) in fixed and permeabilized cells and tissues.

Binding Specificity: Selectively binds F-actin with high affinity.
Spectral Properties: Excitation/emission maxima of approximately 403/427 nm.
Instrument Compatibility: Compatible with 405 nm laser excitation and DAPI fluorescence filter sets.
Applications: Suitable for actin cytoskeleton staining and multicolor fluorescence microscopy.

Fluorescence image of HeLa cells stained with Phalloidin-iFluor® 405 Conjugate using fluorescence microscope. The cells were fixed in 4% formaldehyde, permeabilised with 0.2% Triton X-100 followed by staining with Phalloidin-iFluor® 405 Conjugate (#23111) and counter staining with Nuclear Red™ DCS1 (#17552). The image was acquired with fluorescence microscope using violet and cy5 filters.

Protocol

SDS

Catalog	Size	Price	Quantity
23111	300 Tests	Price

Overview

This blue fluorescent phalloidin conjugate (equivalent to Alexa Fluor® 405-labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Citations

View all 63 citations: Citation Explorer

Dynamic micropatterning uncovers spatial dynamics of B cell immune synapse formation

Authors:

Tejeda-Gonz{\'a}lez, Blanca and Hern{\'a}ndez-P{\'e}rez, Sara and Kiviluoto, Elmeri and Ivaska, Johanna and Mattila, Pieta K

Journal:

Journal of Cell Science (2026): jcs--264876

The embryo game uncovers hidden cell behaviours

Authors:

Abou Chakra, Maria and Hislop, Joshua and Egilmez, Ipek and Alkalai, Rafael and Bashth, Omar and Maheden, Kieran and Gundagathi, Akash and Ebrahimkhani, Mo R and Shakiba, Nika

Journal:

Nature Communications (2026)

Motor activity of nonmuscle myosin 2A is a key component of bipolar filament turnover in cells

Authors:

Chougule, Anil and Svitkina, Tatyana

Journal:

bioRxiv (2026): 2026--03

Proximity labeling of the Listeria monocytogenes surface reveals pathogen control of a host deubiquitinase

Authors:

Lamason, Rebecca and Woida, Patrick J and Smith, Maisie W

Journal:

bioRxiv (2026): 2026--02

Cell cycle-dependent transitions in PCP protein mobility erase and restore planar cell polarity in mitosis

Authors:

Ouyang, Debra and Basta, Lena P and Sil, Parijat and Joyce, Bradley W and Posfai, Eszter and Devenport, Danelle

Journal:

bioRxiv (2026): 2026--01

References

View all 127 references: Citation Explorer

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B.

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Anti-acetylated tubulin antibody staining and phalloidin staining in the starlet sea anemone Nematostella vectensis

Authors:

Genikhovich G, Technau U.

Journal:

Cold Spring Harb Protoc (2009): pdb prot5283

Physical properties

Molecular weight	~1100
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.48
Correction factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000 ¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 2, 2026