Phalloidin iFluor® 660 is an F-actin-specific probe conjugated to the far red-fluorescent dye iFluor® 660. Phalloidin, a bicyclic peptide derived from Amanita phalloides ("death cap" mushroom), is widely used for the selective labeling of filamentous actin (F-actin) in fluorescence microscopy.

The conjugation of phalloidin to iFluor® 660 retains its high binding affinity and specificity for F-actin, providing reliable and reproducible labeling across various biological systems. The iFluor® 660 fluorophore offers exceptional brightness and photostability, enabling prolonged imaging sessions with minimal photobleaching. Additionally, the probe exhibits negligible nonspecific binding, ensuring high-contrast visualization of actin filaments, even in complex biological environments.

Phalloidin iFluor® 660 is optimized for high-resolution imaging and quantitative analysis of F-actin in diverse applications, including fixed tissue sections, cultured cells, and in vitro actin polymerization assays. Its compatibility with multiplex fluorescence imaging allows co-labeling with fluorescent proteins, quantum dots, iFluor® derivatives, and antibody-based detection systems.