Phalloidin Conjugates | AAT Bioquest

Phalloidin-Fluorescein Conjugate

This green fluorescent phalloidin conjugate (equivalent to FITC labeled phalloidin) selectively binds to F-actins. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23101	300 Tests	Price

Citations

View all 49 citations: Citation Explorer

Hypoxia-and Postirradiation reoxygenation-induced HMHA1/ARHGAP45 expression contributes to cancer cell invasion in a HIF-dependent manner

Authors:

Lee, Peter WT and Suwa, Tatsuya and Kobayashi, Minoru and Yang, Hui and Koseki, Lina R and Takeuchi, Satoshi and Chow, Christalle CT and Yasuhara, Takaaki and Harada, Hiroshi

Journal:

British Journal of Cancer (2024): 1--12

MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling

Authors:

MA, Lanfang and WANG, Shuo and YANG, Jun and TANG, Weicheng and WU, Zhangying and CAO, Lili and LUO, Aiyue and FU, Fangfang and YANG, Shuhong and WANG, Shixuan

Journal:

Journal of Reproduction and Development (2023): 2022--137

Sirt1 Regulates Corneal Epithelial Migration by Deacetylating Cortactin

Authors:

Lin, Yong and Liu, Qi and Li, Li and Yang, Rusen and Ye, Juxiu and Yang, Shuai and Luo, Guangying and Reinach, Peter S and Yan, Dongsheng

Journal:

Investigative Ophthalmology \& Visual Science (2022): 14--14

2-Mercaptoethanol promotes porcine oocyte maturation in vitro by maintaining autophagy homeostasis

Authors:

Zhang, Yaping and Li, Qiqi and Li, Wangchang and Yan, Ke and Liu, Yaru and Xu, Huiyan and Jiang, Mingsheng and Lu, Yangqing and Liang, Xingwei and Shang, Jianghua and others,

Journal:

Theriogenology (2022): 155--167

Allicin protects porcine oocytes against LPS-induced defects during maturation in vitro

Authors:

Li, Qiqi and Zhang, Yaping and Li, Wangchang and Yan, Ke and Liu, Yaru and Xu, Huiyan and Lu, Yangqing and Liang, Xingwei and Yang, Xiaogan

Journal:

Theriogenology (2022): 138--147

References

View all 127 references: Citation Explorer

Improved penile histology by phalloidin stain: circular and longitudinal cavernous smooth muscles, dual-endothelium arteries, and erectile dysfunction-associated changes

Authors:

Lin G, Qiu X, F and el TM, Albersen M, Wang Z, Lue TF, Lin CS.

Journal:

Urology (2011): 970 e1

Phalloidin perturbs the interaction of human non-muscle myosin isoforms 2A and 2C1 with F-actin

Authors:

Diensthuber RP, Muller M, Heissler SM, Taft MH, Chizhov I, Manstein DJ.

Journal:

FEBS Lett (2011): 767

pH-(low)-insertion-peptide (pHLIP) translocation of membrane impermeable phalloidin toxin inhibits cancer cell proliferation

Authors:

An M, Wijesinghe D, Andreev OA, Reshetnyak YK, Engelman DM.

Journal:

Proc Natl Acad Sci U S A (2010): 20246

Labeling cytoskeletal F-actin with rhodamine phalloidin or fluorescein phalloidin for imaging

Authors:

Chazotte B., undefined

Journal:

Cold Spring Harb Protoc (2010): pdb prot4947

Effect of Phalloidin on Filaments Polymerized from Heart Muscle Adp-Actin Monomers

Authors:

Vig A, Dudas R, Kupi T, Orban J, Hild G, Lorinczy D, Nyitrai M.

Journal:

J Therm Anal Calorim (2009): 721

Physical properties

Molecular weight	~1100
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 9, 2024