XFD647 Phalloidin equivalent to Alexa Fluor® 647 phalloidin

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Spectral properties

Correction Factor (260 nm)	0.00
Correction Factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Correction Factor (260 nm)

0.00

Correction Factor (280 nm)

0.03

Extinction coefficient (cm ^-1 M ^-1)

239000

Excitation (nm)

650

Emission (nm)

671

Quantum yield

0.33¹

XFD647, produced by AAT Bioquest, shares the identical chemical structure as Alexa Fluor® 647 (Alexa Fluor® being the trademark of ThermoFisher). The XFD647 phalloidin conjugate is essentially equivalent to Alexa Fluor® 647 phalloidin and exhibits a far-red fluorescent signal. This conjugate specifically binds to F-actins, and when used at nanomolar concentrations, it serves as a convenient tool for labeling, identifying, and quantifying F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments. The application of fluorescent phalloidin derivatives, such as XFD647, has proven crucial in the detailed examination of actin networks at high resolution. AAT Bioquest provides a range of fluorescent phalloidin derivatives in different colors to facilitate multicolor imaging applications. They are fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot™ nanocrystals, and other iFluor® conjugates, including secondary antibodies.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.00
Correction Factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
XFD350 Phalloidin XFD350 Same Structure to Alexa Fluor™ 350	343	441	19000	-	0.19
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.11
XFD594 Phalloidin XFD594 Same Structure to Alexa Fluor™ 594	590	618	92000	0.66¹	0.56
XFD647 Azide	650	671	270000	0.33¹	0.03
XFD647 Alkyne	650	671	270000	0.33¹	0.03
XFD647 amine	650	671	270000	0.33¹	0.03
XFD647 acid	650	671	270000	0.33¹	0.03

References

View all 10 references: Citation Explorer

TGFβ2 Induces the Formation of Cross-Linked Actin Networks (CLANs) in Human Trabecular Meshwork Cells Through the Smad and Non-Smad Dependent Pathways.
Authors: Montecchi-Palmer, Michela and Bermudez, Jaclyn Y and Webber, Hannah C and Patel, Gaurang C and Clark, Abbot F and Mao, Weiming
Journal: Investigative ophthalmology & visual science (2017): 1288-1295

Glycation of extracellular matrix proteins impairs migration of immune cells.
Authors: Haucke, Elisa and Navarrete-Santos, Alexander and Simm, Andreas and Silber, Rolf-Edgar and Hofmann, Britt
Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (2014): 239-45

Neuroprotective effects of hypothermia on synaptic actin cytoskeletal changes induced by perinatal asphyxia.
Authors: Muñiz, Javier and Romero, Juan and Holubiec, Mariana and Barreto, George and González, Janneth and Saint-Martin, Madeleine and Blanco, Eduardo and Carlos Cavicchia, Juan and Castilla, Rocío and Capani, Francisco
Journal: Brain research (2014): 81-90

Effects of perinatal asphyxia on rat striatal cytoskeleton.
Authors: Saraceno, G E and Ayala, M V and Badorrey, M S and Holubiec, M and Romero, J I and Galeano, P and Barreto, G and Giraldez-Alvárez, L D and Kölliker-Fres, R and Coirini, H and Capani, F
Journal: Synapse (New York, N.Y.) (2012): 9-19

Cross-linked actin networks (CLANs) are present in lamina cribrosa cells.
Authors: Job, Raly and Raja, Vignesh and Grierson, Ian and Currie, Laura and O'Reilly, Steven and Pollock, Natalie and Knight, Emma and Clark, Abbot F
Journal: The British journal of ophthalmology (2010): 1388-92

[Uptake and accumulation of gentamicin by mouse hair cells from the cochlear explants in vitro].
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2008): 439-42

Incidence of protein on actin bridges between endothelium and smooth muscle in arterioles demonstrates heterogeneous connexin expression and phosphorylation.
Authors: Isakson, Brant E and Best, Angela K and Duling, Brian R
Journal: American journal of physiology. Heart and circulatory physiology (2008): H2898-904

Depolymerisation and rearrangement of actin filaments during exocytosis in rat peritoneal mast cells: involvement of ryanodine-sensitive calcium stores.
Authors: Guzmán, R E and Bolaños, P and Delgado, A and Rojas, H and DiPolo, R and Caputo, C and Jaffe, E H
Journal: Pflugers Archiv : European journal of physiology (2007): 131-41

[Distribution of gentamicin in inner ear after intratympanic gentamicin injection].
Authors: Liu, Jian-Ping and Dai, Chun-Fu and Wang, Zheng-Min and Chi, Fang-Lu and Tian, Jie and Da, Cui-Di
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2006): 851-6

The protein encoded by the US3 orthologue of Marek's disease virus is required for efficient de-envelopment of perinuclear virions and involved in actin stress fiber breakdown.
Authors: Schumacher, Daniel and Tischer, B Karsten and Trapp, Sascha and Osterrieder, Nikolaus
Journal: Journal of virology (2005): 3987-97